Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir.

@article{citeulike:2859678, abstract = {The complete genome sequence of Geobacillus thermodenitrificans NG80-2, a thermophilic bacillus isolated from a deep oil reservoir in Northern China, consists of a 3,550,319-bp chromosome and a 57,693-bp plasmid. The genome reveals that NG80-2 is well equipped for adaptation into a wide variety of environmental niches, including oil reservoirs, by possessing genes for utilization of a broad range of energy sources, genes encoding various transporters for efficient nutrient uptake and detoxification, and genes for a flexible respiration system including an aerobic branch comprising five terminal oxidases and an anaerobic branch comprising a complete denitrification pathway for quick response to dissolved oxygen fluctuation. The identification of a nitrous oxide reductase gene has not been previously described in Gram-positive bacteria. The proteome further reveals the presence of a long-chain alkane degradation pathway; and the function of the key enzyme in the pathway, the long-chain alkane monooxygenase LadA, is confirmed by in vivo and in vitro experiments. The thermophilic soluble monomeric LadA is an ideal candidate for treatment of environmental oil pollutions and biosynthesis of complex molecules.}, address = {TEDA School of Biological Sciences and Biotechnology, Nankai University, Tianjin 300457, China.}, author = {Feng, L. and Wang, W. and Cheng, J. and Ren, Y. and Zhao, G. and Gao, C. and Tang, Y. and Liu, X. and Han, W. and Peng, X. and Liu, R. and Wang, L. }, citeulike-article-id = {2859678}, doi = {http://dx.doi.org/10.1073/pnas.0609650104}, issn = {0027-8424}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {alkane, genome, oxygenase, proteome}, month = {March}, number = {13}, pages = {5602--5607}, posted-at = {2008-06-03 19:15:23}, priority = {2}, title = {Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir.}, url = {http://dx.doi.org/10.1073/pnas.0609650104}, volume = {104}, year = {2007} }

TY - JOUR ID - citeulike:2859678 L3 - citeulike-article-id:2859678 TI - Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir. JF - Proceedings of the National Academy of Sciences of the United States of America VL - 104 IS - 13 SP - 5602 EP - 5607 AD - TEDA School of Biological Sciences and Biotechnology, Nankai University, Tianjin 300457, China. SN - 0027-8424 N2 - The complete genome sequence of Geobacillus thermodenitrificans NG80-2, a thermophilic bacillus isolated from a deep oil reservoir in Northern China, consists of a 3,550,319-bp chromosome and a 57,693-bp plasmid. The genome reveals that NG80-2 is well equipped for adaptation into a wide variety of environmental niches, including oil reservoirs, by possessing genes for utilization of a broad range of energy sources, genes encoding various transporters for efficient nutrient uptake and detoxification, and genes for a flexible respiration system including an aerobic branch comprising five terminal oxidases and an anaerobic branch comprising a complete denitrification pathway for quick response to dissolved oxygen fluctuation. The identification of a nitrous oxide reductase gene has not been previously described in Gram-positive bacteria. The proteome further reveals the presence of a long-chain alkane degradation pathway; and the function of the key enzyme in the pathway, the long-chain alkane monooxygenase LadA, is confirmed by in vivo and in vitro experiments. The thermophilic soluble monomeric LadA is an ideal candidate for treatment of environmental oil pollutions and biosynthesis of complex molecules. KW - alkane KW - genome KW - oxygenase KW - proteome AU - Feng, L AU - Wang, W AU - Cheng, J AU - Ren, Y AU - Zhao, G AU - Gao, C AU - Tang, Y AU - Liu, X AU - Han, W AU - Peng, X AU - Liu, R AU - Wang, L PY - 2007/03/27/ UR - http://dx.doi.org/10.1073/pnas.0609650104 ER -