Molecular dissection of Penelope transposable element regulatory machinery

@article{citeulike:2527545, abstract = {Penelope-like elements (PLEs) represent a new class of retroelements identified in more than 80 species belonging to at least 10 animal phyla. Penelope isolated from Drosophila virilis is the only known transpositionally active representative of this class. Although the size and structure of the Penelope major transcript has been previously described in both D. virilis and D. melanogaster transgenic strains, the architecture of the Penelope regulatory region remains unknown. In order to determine the localization of presumptive Penelope promoter and enhancer-like elements, segments of the putative Penelope regulatory region were linked to a CAT reporter gene and introduced into D. melanogaster by P-element-mediated transformation. The results obtained using ELISA to measure CAT expression levels and RNA studies, including RTPCR, suggest that the active Penelope transposon contains an internal promoter similar to the TATA-less promoters of LINEs. The results also suggest that some of the Penelope regulatory sequences control the preferential expression in the ovaries of the adult flies by enhancing expression in the ovary and reducing expression in the carcass. The possible significance of the intron within Penelope for the function and evolution of PLEs, and the effect of Penelope insertions on adjacent genes, are discussed. 10.1093/nar/gkm1166}, author = {Schostak, Nataliya and Pyatkov, Konstantin and Zelentsova, Elena and Arkhipova, Irina and Shagin, Dmitrii and Shagina, Irina and Mudrik, Elena and Blintsov, Anatolii and Clark, Ivan and Finnegan, David J. and Evgen'ev, Michael }, citeulike-article-id = {2527545}, doi = {10.1093/nar/gkm1166}, journal = {Nucl. Acids Res.}, keywords = {te}, month = {March}, pages = {gkm1166+}, posted-at = {2008-05-07 09:55:17}, priority = {2}, title = {Molecular dissection of Penelope transposable element regulatory machinery}, url = {http://dx.doi.org/10.1093/nar/gkm1166}, year = {2008} }

TY - JOUR ID - citeulike:2527545 L3 - citeulike-article-id:2527545 TI - Molecular dissection of Penelope transposable element regulatory machinery JF - Nucl. Acids Res. SP - gkm1166 N2 - Penelope-like elements (PLEs) represent a new class of retroelements identified in more than 80 species belonging to at least 10 animal phyla. Penelope isolated from Drosophila virilis is the only known transpositionally active representative of this class. Although the size and structure of the Penelope major transcript has been previously described in both D. virilis and D. melanogaster transgenic strains, the architecture of the Penelope regulatory region remains unknown. In order to determine the localization of presumptive Penelope promoter and enhancer-like elements, segments of the putative Penelope regulatory region were linked to a CAT reporter gene and introduced into D. melanogaster by P-element-mediated transformation. The results obtained using ELISA to measure CAT expression levels and RNA studies, including RTPCR, suggest that the active Penelope transposon contains an internal promoter similar to the TATA-less promoters of LINEs. The results also suggest that some of the Penelope regulatory sequences control the preferential expression in the ovaries of the adult flies by enhancing expression in the ovary and reducing expression in the carcass. The possible significance of the intron within Penelope for the function and evolution of PLEs, and the effect of Penelope insertions on adjacent genes, are discussed. 10.1093/nar/gkm1166 KW - te AU - Schostak, Nataliya AU - Pyatkov, Konstantin AU - Zelentsova, Elena AU - Arkhipova, Irina AU - Shagin, Dmitrii AU - Shagina, Irina AU - Mudrik, Elena AU - Blintsov, Anatolii AU - Clark, Ivan AU - Finnegan, David AU - Evgen'ev, Michael PY - 2008/03/04/ UR - http://dx.doi.org/10.1093/nar/gkm1166 ER -