Retrotransposon Tf1 is targeted to Pol II promoters by transcription activators.

@article{citeulike:2782543, abstract = {The LTR-retrotransposon Tf1 preserves the coding capacity of its host Schizosaccharomyces pombe by integrating upstream of open reading frames (ORFs). To determine which features of the target sites were recognized by the transposon, we introduced plasmids containing candidate insertion sites into S. pombe and mapped the positions of integration. We found that Tf1 was targeted specifically to the promoters of Pol II-transcribed genes. A detailed analysis of integration in plasmids that contained either ade6 or fbp1 revealed insertions occurred in the promoters at positions where transcription factors bound. Further experiments revealed that the activator Atf1p and its binding site were required for directing integration to the promoter of fbp1. An interaction between Tf1 integrase and Atf1p was observed, indicating that integration at fbp1 was mediated by the activator bound to its promoter. Surprisingly, we found Tf1 contained sequences that activated transcription, and these substituted for elements of the ade6 promoter disrupted by integration.}, address = {Section on Eukaryotic Transposable Elements, Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.}, author = {Leem, Y. E. and Ripmaster, T. L. and Kelly, F. D. and Ebina, H. and Heincelman, M. E. and Zhang, K. and Grewal, S. I. and Hoffman, C. S. and Levin, H. L. }, citeulike-article-id = {2782543}, doi = {10.1016/j.molcel.2008.02.016}, issn = {1097-4164}, journal = {Molecular cell}, keywords = {cis-regulatory, expression, fungi}, month = {April}, number = {1}, pages = {98--107}, posted-at = {2008-05-10 09:06:08}, priority = {2}, title = {Retrotransposon Tf1 is targeted to Pol II promoters by transcription activators.}, url = {http://dx.doi.org/10.1016/j.molcel.2008.02.016}, volume = {30}, year = {2008} }

TY - JOUR ID - citeulike:2782543 L3 - citeulike-article-id:2782543 TI - Retrotransposon Tf1 is targeted to Pol II promoters by transcription activators. JF - Molecular cell VL - 30 IS - 1 SP - 98 EP - 107 AD - Section on Eukaryotic Transposable Elements, Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA. SN - 1097-4164 N2 - The LTR-retrotransposon Tf1 preserves the coding capacity of its host Schizosaccharomyces pombe by integrating upstream of open reading frames (ORFs). To determine which features of the target sites were recognized by the transposon, we introduced plasmids containing candidate insertion sites into S. pombe and mapped the positions of integration. We found that Tf1 was targeted specifically to the promoters of Pol II-transcribed genes. A detailed analysis of integration in plasmids that contained either ade6 or fbp1 revealed insertions occurred in the promoters at positions where transcription factors bound. Further experiments revealed that the activator Atf1p and its binding site were required for directing integration to the promoter of fbp1. An interaction between Tf1 integrase and Atf1p was observed, indicating that integration at fbp1 was mediated by the activator bound to its promoter. Surprisingly, we found Tf1 contained sequences that activated transcription, and these substituted for elements of the ade6 promoter disrupted by integration. KW - cis-regulatory KW - expression KW - fungi AU - Leem, YE AU - Ripmaster, TL AU - Kelly, FD AU - Ebina, H AU - Heincelman, ME AU - Zhang, K AU - Grewal, SI AU - Hoffman, CS AU - Levin, HL PY - 2008/04/11/ UR - http://dx.doi.org/10.1016/j.molcel.2008.02.016 ER -