From the Cover: Combinatorial antibody libraries from survivors of the Turkish H5N1 avian influenza outbreak reveal virus neutralization strategies

@article{citeulike:2729300, abstract = {The widespread incidence of H5N1 influenza viruses in bird populations poses risks to human health. Although the virus has not yet adapted for facile transmission between humans, it can cause severe disease and often death. Here we report the generation of combinatorial antibody libraries from the bone marrow of five survivors of the recent H5N1 avian influenza outbreak in Turkey. To date, these libraries have yielded >300 unique antibodies against H5N1 viral antigens. Among these antibodies, we have identified several broadly reactive neutralizing antibodies that could be used for passive immunization against H5N1 virus or as guides for vaccine design. The large number of antibodies obtained from these survivors provide a detailed immunochemical analysis of individual human solutions to virus neutralization in the setting of an actual virulent influenza outbreak. Remarkably, three of these antibodies neutralized both H1 and H5 subtype influenza viruses. 10.1073/pnas.0801367105}, author = {Kashyap, Arun K. and Steel, John and Oner, Ahmet F. and Dillon, Michael A. and Swale, Ryann E. and Wall, Katherine M. and Perry, Kimberly J. and Faynboym, Aleksandr and Ilhan, Mahmut and Horowitz, Michael and Horowitz, Lawrence and Palese, Peter and Bhatt, Ramesh R. and Lerner, Richard A. }, citeulike-article-id = {2729300}, doi = {10.1073/pnas.0801367105}, journal = {Proceedings of the National Academy of Sciences}, keywords = {influenza, neutralization}, month = {April}, number = {16}, pages = {5986--5991}, posted-at = {2008-04-28 10:02:56}, priority = {2}, title = {From the Cover: Combinatorial antibody libraries from survivors of the Turkish H5N1 avian influenza outbreak reveal virus neutralization strategies}, url = {http://dx.doi.org/10.1073/pnas.0801367105}, volume = {105}, year = {2008} }

TY - JOUR ID - citeulike:2729300 L3 - citeulike-article-id:2729300 TI - From the Cover: Combinatorial antibody libraries from survivors of the Turkish H5N1 avian influenza outbreak reveal virus neutralization strategies JF - Proceedings of the National Academy of Sciences VL - 105 IS - 16 SP - 5986 EP - 5991 N2 - The widespread incidence of H5N1 influenza viruses in bird populations poses risks to human health. Although the virus has not yet adapted for facile transmission between humans, it can cause severe disease and often death. Here we report the generation of combinatorial antibody libraries from the bone marrow of five survivors of the recent H5N1 avian influenza outbreak in Turkey. To date, these libraries have yielded >300 unique antibodies against H5N1 viral antigens. Among these antibodies, we have identified several broadly reactive neutralizing antibodies that could be used for passive immunization against H5N1 virus or as guides for vaccine design. The large number of antibodies obtained from these survivors provide a detailed immunochemical analysis of individual human solutions to virus neutralization in the setting of an actual virulent influenza outbreak. Remarkably, three of these antibodies neutralized both H1 and H5 subtype influenza viruses. 10.1073/pnas.0801367105 KW - influenza KW - neutralization AU - Kashyap, Arun AU - Steel, John AU - Oner, Ahmet AU - Dillon, Michael AU - Swale, Ryann AU - Wall, Katherine AU - Perry, Kimberly AU - Faynboym, Aleksandr AU - Ilhan, Mahmut AU - Horowitz, Michael AU - Horowitz, Lawrence AU - Palese, Peter AU - Bhatt, Ramesh AU - Lerner, Richard PY - 2008/04/22/ UR - http://dx.doi.org/10.1073/pnas.0801367105 ER -