Darwinian Evolution on a Chip

by: Brian M Paegel, Gerald F Joyce

PLoS Biology, Vol. 6, No. 4. (1 April 2008), e85.

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Abstract

Computer control of Darwinian evolution has been demonstrated by propagating a population of RNA enzymes in a microfluidic device. The RNA population was challenged to catalyze the ligation of an oligonucleotide substrate under conditions of progressively lower substrate concentrations. A microchip-based serial dilution circuit automated an exponential growth phase followed by a 10-fold dilution, which was repeated for 500 log-growth iterations. Evolution was observed in real time as the population adapted and achieved progressively faster growth rates over time. The final evolved enzyme contained a set of 11 mutations that conferred a 90-fold improvement in substrate utilization, coinciding with the applied selective pressure. This system reduces evolution to a microfluidic algorithm, allowing the experimenter to observe and manipulate adaptation.

@article{citeulike:2642457, abstract = {Computer control of Darwinian evolution has been demonstrated by propagating a population of RNA enzymes in a microfluidic device. The RNA population was challenged to catalyze the ligation of an oligonucleotide substrate under conditions of progressively lower substrate concentrations. A microchip-based serial dilution circuit automated an exponential growth phase followed by a 10-fold dilution, which was repeated for 500 log-growth iterations. Evolution was observed in real time as the population adapted and achieved progressively faster growth rates over time. The final evolved enzyme contained a set of 11 mutations that conferred a 90-fold improvement in substrate utilization, coinciding with the applied selective pressure. This system reduces evolution to a microfluidic algorithm, allowing the experimenter to observe and manipulate adaptation.}, author = {Paegel, Brian M. and Joyce, Gerald F. }, citeulike-article-id = {2642457}, doi = {10.1371/journal.pbio.0060085}, journal = {PLoS Biology}, keywords = {directed\_evolution, journal\_club}, month = {April}, number = {4}, pages = {e85+}, posted-at = {2008-04-29 20:13:18}, priority = {4}, title = {Darwinian Evolution on a Chip}, url = {http://dx.doi.org/10.1371/journal.pbio.0060085}, volume = {6}, year = {2008} }

RIS record

TY - JOUR ID - citeulike:2642457 L3 - citeulike-article-id:2642457 TI - Darwinian Evolution on a Chip JF - PLoS Biology VL - 6 IS - 4 SP - e85 N2 - Computer control of Darwinian evolution has been demonstrated by propagating a population of RNA enzymes in a microfluidic device. The RNA population was challenged to catalyze the ligation of an oligonucleotide substrate under conditions of progressively lower substrate concentrations. A microchip-based serial dilution circuit automated an exponential growth phase followed by a 10-fold dilution, which was repeated for 500 log-growth iterations. Evolution was observed in real time as the population adapted and achieved progressively faster growth rates over time. The final evolved enzyme contained a set of 11 mutations that conferred a 90-fold improvement in substrate utilization, coinciding with the applied selective pressure. This system reduces evolution to a microfluidic algorithm, allowing the experimenter to observe and manipulate adaptation. KW - directed_evolution KW - journal_club AU - Paegel, Brian AU - Joyce, Gerald PY - 2008/04/01/ UR - http://dx.doi.org/10.1371/journal.pbio.0060085 ER -

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