Wed, 20 Aug 2008 22:10:15 BST CiteULike: jyuh Leo http://www.citeulike.org/user/jyuh/author/Leo CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/user/jyuh/article/2792223 <i>Science, Vol. 320, No. 5877. (9 May 2008), pp. 807-811.</i><br /><br />To understand the system of secreted proteins and receptors involved in cell-cell signaling, we produced a comprehensive set of recombinant secreted proteins and the extracellular domains of transmembrane proteins, which constitute most of the protein components of the extracellular space. Each protein was tested in a suite of assays that measured metabolic, growth, or transcriptional responses in diverse cell types. The pattern of responses across assays was analyzed for the degree of functional selectivity of each protein. One of the highly selective proteins was a previously undescribed ligand, designated interleukin-34 (IL-34), which stimulates monocyte viability but does not affect responses in a wide spectrum of other assays. In a separate functional screen, we used a collection of extracellular domains of transmembrane proteins to discover the receptor for IL-34, which was a known cytokine receptor, colony-stimulating factor 1 (also called macrophage colony-stimulating factor) receptor. This systematic approach is thus useful for discovering new ligands and receptors and assessing the functional selectivity of extracellular regulatory proteins. 10.1126/science.1154370 Discovery of a Cytokine and Its Receptor by Functional Screening of the Extracellular Proteome Haishan Lin Ernestine Lee Kevin Hestir Cindy Leo Minmei Huang Elizabeth Bosch Robert Halenbeck Ge Wu Aileen Zhou Dirk Behrens Diane Hollenbaugh Thomas Linnemann Minmin Qin Justin Wong Keting Chu Stephen Doberstein Lewis Williams doi:10.1126/science.1154370 Science, Vol. 320, No. 5877. (9 May 2008), pp. 807-811. 2008-05-13T01:59:36-00:00 2008 Science 320 5877 807 811 cytokine method proteomics http://www.citeulike.org/user/jyuh/article/692112 <i>Twin Research and Human Genetics, Vol. 9, No. 3. (June 2006), pp. 334-342.</i> Fitting Genetic Models Using Markov Chain Monte Carlo Algorithms With BUGS Van den Berg M Stephanie Beem Leo Boomsma I Dorret doi:10.1375/183242706777591399 Twin Research and Human Genetics, Vol. 9, No. 3. (June 2006), pp. 334-342. 2006-06-11T04:47:42-00:00 2006 Twin Research and Human Genetics 1832-4274 9 3 334 342 Australian Academic Press no-tag http://www.citeulike.org/user/jyuh/article/692113 <i>Twin Research and Human Genetics, Vol. 9, No. 3. (June 2006), pp. 325-333.</i> Implementation of a Combined Association-Linkage Model for Quantitative Traits in Linear Mixed Model Procedures of Statistical Packages Beem A Leo Boomsma I Dorret doi:10.1375/183242706777591272 Twin Research and Human Genetics, Vol. 9, No. 3. (June 2006), pp. 325-333. 2006-06-11T04:47:42-00:00 2006 Twin Research and Human Genetics 1832-4274 9 3 325 333 Australian Academic Press genetics heritability mixed-model