Thu, 21 Aug 2008 02:24:14 BST CiteULike: vrich Short http://www.citeulike.org/user/vrich/author/Short CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/user/vrich/article/700422 <i>Science, Vol. 308, No. 5721. (22 April 2005), pp. 554-557.</i><br /><br />The species complexity of microbial communities and challenges in culturing representative isolates make it difficult to obtain assembled genomes. Here we characterize and compare the metabolic capabilities of terrestrial and marine microbial communities using largely unassembled sequence data obtained by shotgun sequencing DNA isolated from the various environments. Quantitative gene content analysis reveals habitat-specific fingerprints that reflect known characteristics of the sampled environments. The identification of environment-specific genes through a gene-centric comparative analysis presents new opportunities for interpreting and diagnosing environments. 10.1126/science.1107851 Comparative Metagenomics of Microbial Communities Susannah Tringe Christian von Mering Arthur Kobayashi Asaf Salamov Kevin Chen Hwai Chang Mircea Podar Jay Short Eric Mathur John Detter Peer Bork Philip Hugenholtz Edward Rubin doi:10.1126/science.1107851 Science, Vol. 308, No. 5721. (22 April 2005), pp. 554-557. 2006-06-19T04:39:47-00:00 2005 Science 308 5721 554 557 community_genomics silage whale http://www.citeulike.org/user/vrich/article/300019 <i>Science, Vol. 309, No. 5738. (19 August 2005), pp. 1242-1245.</i><br /><br />The SAR11 clade consists of very small, heterotrophic marine alpha-proteobacteria that are found throughout the oceans, where they account for about 25% of all microbial cells. Pelagibacter ubique, the first cultured member of this clade, has the smallest genome and encodes the smallest number of predicted open reading frames known for a free-living microorganism. In contrast to parasitic bacteria and archaea with small genomes, P. ubique has complete biosynthetic pathways for all 20 amino acids and all but a few cofactors. P. ubique has no pseudogenes, introns, transposons, extrachromosomal elements, or inteins; few paralogs; and the shortest intergenic spacers yet observed for any cell. Genome streamlining in a cosmopolitan oceanic bacterium. SJ Giovannoni HJ Tripp S Givan M Podar KL Vergin D Baptista L Bibbs J Eads TH Richardson M Noordewier MS Rappé JM Short JC Carrington EJ Mathur doi:10.1126/science.1114057 Science, Vol. 309, No. 5738. (19 August 2005), pp. 1242-1245. 2005-08-21T11:53:02-00:00 2005 Science 1095-9203 309 5738 1242 1245 sar11