Thu, 21 Aug 2008 01:17:03 BST CiteULike: xico fiber http://www.citeulike.org/user/xico/tag/fiber CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/user/xico/article/2800291 <i>FEBS letters, Vol. 581, No. 16. (26 June 2007), pp. 2929-2934.</i><br /><br />Cannabidiolic-acid (CBDA) synthase is the enzyme that catalyzes oxidative cyclization of cannabigerolic-acid into CBDA, the dominant cannabinoid constituent of the fiber-type Cannabis sativa. We cloned a novel cDNA encoding CBDA synthase by reverse transcription and polymerase chain reactions with degenerate and gene-specific primers. Biochemical characterization of the recombinant enzyme demonstrated that CBDA synthase is a covalently flavinylated oxidase. The structural and functional properties of CBDA synthase are quite similar to those of tetrahydrocannabinolic-acid (THCA) synthase, which is responsible for the biosynthesis of THCA, the major cannabinoid in drug-type Cannabis plants. Cannabidiolic-acid synthase, the chemotype-determining enzyme in the fiber-type Cannabis sativa. F Taura S Sirikantaramas Y Shoyama K Yoshikai Y Shoyama S Morimoto doi:10.1016/j.febslet.2007.05.043 FEBS letters, Vol. 581, No. 16. (26 June 2007), pp. 2929-2934. 2008-05-14T23:34:48-00:00 2007 FEBS letters 0014-5793 581 16 2929 2934 acid cannabidiol cannabidiolic cannabis cbd-synthase cdb chemotype fiber hemp marijuana psychoactivity sativa synthase thc