Mon, 07 Jul 2008 16:45:41 BST CiteULike: xingxu Cheung http://www.citeulike.org/user/xingxu/author/Cheung CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/user/xingxu/article/1336057 <i>Science (17 May 2007), 1138341.</i><br /><br />Significant fractions of eukaryotic genomes give rise to RNA, much of which is unannotated and has reduced protein-coding potential. The genomic origins and the relations of human nuclear and cytosolic polyadenylated RNAs longer than 200 nucleotides and whole-cell RNAs less than 200 nt are investigated in this genome-wide study. Subcellular addresses for nucleotides present in detected RNAs were assigned, and their potential processing into short RNAs was investigated. Taken together, these observations suggest a role for some unannotated RNAs as primary transcripts for the production of short RNAs. Three novel potentially functional classes of RNAs have been identified, two of which are syntenically conserved and correlate with the expression state of protein-coding genes. These data support a highly interleaved organization of the human transcriptome. 10.1126/science.1138341 RNA Maps Reveal New RNA Classes and a Possible Function for Pervasive Transcription Philipp Kapranov Jill Cheng Sujit Dike David Nix Radharani Duttagupta Aarron Willingham Peter Stadler Jana Hertel Joerg Hackermueller Ivo Hofacker Ian Bell Evelyn Cheung Jorg Drenkow Erica Dumais Sandeep Patel Gregg Helt Madhavan Ganesh Srinka Ghosh Antonio Piccolboni Victor Sementchenko Hari Tammana Thomas Gingeras doi:10.1126/science.1138341 Science (17 May 2007), 1138341. 2007-05-27T00:42:53-00:00 2007 Science 1138341 breakthrough methods transcriptome http://www.citeulike.org/user/xingxu/article/1043226 <i>Nat Genet (7 January 2007)</i><br /><br />Variation in DNA sequence contributes to individual differences in quantitative traits, but in humans the specific sequence variants are known for very few traits. We characterized variation in gene expression in cells from individuals belonging to three major population groups. This quantitative phenotype differs significantly between European-derived and Asian-derived populations for 1,097 of 4,197 genes tested. For the phenotypes with the strongest evidence of cis determinants, most of the variation is due to allele frequency differences at cis-linked regulators. The results show that specific genetic variation among populations contributes appreciably to differences in gene expression phenotypes. Populations differ in prevalence of many complex genetic diseases, such as diabetes and cardiovascular disease. As some of these are probably influenced by the level of gene expression, our results suggest that allele frequency differences at regulatory polymorphisms also account for some population differences in prevalence of complex diseases. Common genetic variants account for differences in gene expression among ethnic groups. Richard S Spielman Laurel A Bastone Joshua T Burdick Michael Morley Warren J Ewens Vivian G Cheung doi:10.1038/ng1955 Nat Genet (7 January 2007) 2007-01-15T20:02:13-00:00 2007 Nat Genet 1061-4036 decode hapmap