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Ключевое слово cell_free [29 articles]

Recent papers classified by the tag cell_free.
  • Amino acid stabilization for cell-free protein synthesis by modification of the Escherichia coli genome.
    Metab Eng, Vol. 6, No. 3. (July 2004), pp. 197-203.
    posted to cell_free by viktor21614 on 2008-07-24 08:36:09 as ** along with 2 people JeremyZucker Odi
  • Expression of soluble recombinant proteins in a cell-free system using a 96-well format
    Journal of Biochemical and Biophysical Methods, Vol. 55, No. 3. (28 March 2003), pp. 233-240.
    by Didier Busso, Rosalind Kim, Sung-Hou Kim
    posted to 96-well cell_free by viktor21614 on 2008-07-22 16:14:16 as **
  • Cell-free synthesis and amino acid-selective stable isotope labeling of proteins for NMR analysis
    Journal of Biomolecular NMR, Vol. 6, No. 2. (1995), pp. 129-134.
    by Takanori Kigawa, Yutaka Muto, Shigeyuki Yokoyama
    posted to cell_free labeling nmr by viktor21614 on 2008-07-24 08:02:23 as **
  • Cell-free expression and selective isotope labelling in protein NMR
    Magnetic Resonance in Chemistry, Vol. 44, No. S1. (2006), pp. S2-S9.
    by David Staunton, Robin Schlinkert, Giulia Zanetti, Simon A Colebrook, Iain D Campbell
    posted to cell_free labeling nmr by viktor21614 on 2008-08-28 15:02:26 as **
  • A modified procedure for fast purification of T7 RNA polymerase.
    Protein Expr Purif, Vol. 16, No. 2. (July 1999), pp. 355-358.
    by Y Li, E Wang, Y Wang
    posted to t7_rna_polymerase cell_free by viktor21614 on 2008-09-03 14:32:47 as ** along with 1 person Odi
  • Optimization of an Escherichia coli system for cell-free synthesis of selectively N-labelled proteins for rapid analysis by NMR spectroscopy.
    Eur J Biochem, Vol. 271, No. 20. (October 2004), pp. 4084-4093.
    by K Ozawa, MJ Headlam, PM Schaeffer, BR Henderson, NE Dixon, G Otting
    posted to cell_free ecoli labeling nmr by viktor21614 on 2008-07-24 08:21:33 as ** along with 2 people dayjm Odi
  • Cell-free production and stable-isotope labeling of milligram quantities of proteins
    FEBS Letters, Vol. 442, No. 1. (8 January 1999), pp. 15-19.
    by Takanori Kigawa, Takashi Yabuki, Yasuhiko Yoshida, Michio Tsutsui, Yutaka Ito, Takehiko Shibata, Shigeyuki Yokoyama
    posted to cell_free labeling by viktor21614 on 2008-07-22 16:13:00 as **
  • A continuous cell-free protein synthesis system for coupled transcription-translation.
    J Biochem (Tokyo), Vol. 110, No. 2. (August 1991), pp. 166-168.
    posted to cell_free continous by viktor21614 on 2008-07-24 07:55:16 as ** along with 1 person Odi
  • Cell-free expression as an emerging technique for the large scale production of integral membrane protein
    FEBS Journal, Vol. 273, No. 18. (September 2006), pp. 4141-4153.
    posted to cell_free membrane by viktor21614 on 2007-08-16 16:08:07 as ** along with 1 person Odi
  • Improvement of Escherichia coli cell-free system by utilization of cell extract having additional property. Problems and countermeasures.
    Appl Biochem Biotechnol, Vol. 53, No. 1. (April 1995), pp. 29-35.
    posted to cell_free by viktor21614 on 2008-07-24 07:10:33 as ** along with 1 person Odi
  • Insertion of Membrane Proteins into Discoidal Membranes Using a Cell-Free Protein Expression Approach
    J. Proteome Res., Vol. 7, No. 8. (1 August 2008), pp. 3535-3542.
    by Federico Katzen, Julia E Fletcher, Jian-Ping Yang, Douglas Kang, Todd C Peterson, Jenny A Cappuccio, Craig D Blanchette, Todd Sulchek, Brett A Chromy, Paul D Hoeprich, Matthew A Coleman, Wieslaw Kudlicki
  • Cell-free expression and functional reconstitution of CALM in clathrin assembly.
    Exp Mol Med, Vol. 33, No. 2. (30 June 2001), pp. 89-94.
    by JA Kim, HL Kim
    posted to cell_free by viktor21614 on 2008-07-24 08:37:45 as ** along with 1 person Odi
  • Evaluation of detergents for the soluble expression of alpha-helical and beta-barrel-type integral membrane proteins by a preparative scale individual cell-free expression system.
    FEBS J, Vol. 272, No. 23. (December 2005), pp. 6024-6038.
    posted to cell_free detergent membrane_protein by viktor21614 on 2008-05-06 17:19:47 as ** along with 1 person Odi
  • Enhanced cell-free protein synthesis using a S30 extract from Escherichia coli grown rapidly at 42 degrees C in an amino acid enriched medium.
    Biotechnol Prog, Vol. 21, No. 2. (r 2005), pp. 608-613.
    by T Yamane, Y Ikeda, T Nagasaka, H Nakano
    posted to cell_free ecoli s30 by viktor21614 on 2008-07-22 15:38:23 as ** along with 1 person Odi
  • Screening for soluble expression constructs using cell-free protein synthesis.
    Int J Biol Macromol (6 April 2006)
    by T Lamla, S Hoerer, M M T M Bauer
    posted to cell_free by viktor21614 on 2008-07-22 15:31:02 as ** along with 1 person Odi
  • Prolonging cell-free protein synthesis with a novel ATP regeneration system
    Biotechnology and Bioengineering, Vol. 66, No. 3. (1999), pp. 180-188.
    by Dong-Myung Kim, James R Swartz
    posted to cell_free by viktor21614 on 2008-07-24 08:19:52 as **
  • A cell-free protein synthesis system for high-throughput proteomics.
    Proceedings of the National Acadaemy of Science USA, Vol. 99, No. 23. (12 November 2002), pp. 14652-14657.
    posted to cell_free by viktor21614 on 2008-07-24 08:41:04 as ** along with 1 person Odi
  • High level cell-free expression and specific labeling of integral membrane proteins
    European Journal of Biochemistry, Vol. 271, No. 3. (2004), pp. 568-580.
    by Christian Klammt, Frank Löhr, Birgit Schäfer, Winfried Haase, Volker Dötsch, Heinz Rüterjans, Clemens Glaubitz, Frank Bernhard
    posted to cell_free membrane_protein by viktor21614 on 2008-07-22 15:28:14 as **
  • Preparative scale cell-free expression systems: New tools for the large scale preparation of integral membrane proteins for functional and structural studies.
    Methods (26 August 2006)
    by Daniel Schwarz, Christian Klammt, Alexander Koglin, Frank Löhr, Birgit Schneider, Volker Dötsch, Frank Bernhard
    posted to cell_free membrane_protein by viktor21614 on 2008-07-24 08:46:22 as ** along with 3 people Odi jyuh rph
  • A bilayer cell-free protein synthesis system for high-throughput screening of gene products
    FEBS Letters, Vol. 514, No. 1. (6 March 2002), pp. 102-105.
    by Tatsuya Sawasaki, Yoshinori Hasegawa, Masateru Tsuchimochi, Nami Kamura, Tomio Ogasawara, Toshihiro Kuroita, Yaeta Endo
    posted to cell_free by viktor21614 on 2008-07-22 16:19:29 as **
  • High efficiency cell-free synthesis of proteins: Refinement of the coupled transcription/translation system
    Analytical Biochemistry, Vol. 206, No. 2. (1 November 1992), pp. 389-393.
    by Wieslaw Kudlicki, Gisela Kramer, Boyd Hardesty
    posted to cell_free by viktor21614 on 2008-07-24 07:58:47 as **
  • Cell-free production of aggregation-prone proteins in soluble and active forms.
    Biotechnol Prog, Vol. 21, No. 5. (t 2005), pp. 1412-1419.
    by SH Kang, DM Kim, HJ Kim, SY Jun, KY Lee, HJ Kim
    posted to aggregation cell_free by viktor21614 on 2008-07-24 07:56:46 as ** along with 1 person Odi
  • Effects of growth rate on cell extract performance in cell-free protein synthesis.
    Biotechnol Bioeng (3 May 2006)
    by James Zawada, James Swartz
    posted to cell_free by viktor21614 on 2008-07-24 07:07:10 as ** along with 1 person Odi
  • Simple procedures for the construction of a robust and cost-effective cell-free protein synthesis system.
    J Biotechnol (27 May 2006)
    by Tae-Wan W Kim, Jung-Won W Keum, In-Seok S Oh, Cha-Yong Y Choi, Chang-Gil G Park, Dong-Myung M Kim
    posted to cell_free by viktor21614 on 2008-07-22 15:56:28 as ** along with 1 person Odi
  • Streamlining Escherichia coli S30 extract preparation for economical cell-free protein synthesis.
    Biotechnol Prog, Vol. 21, No. 2. (r 2005), pp. 460-465.
    by DV Liu, JF Zawada, JR Swartz
    posted to cell_free s30 by viktor21614 on 2008-07-22 15:57:15 as ** along with 2 people dayjm Odi
  • Preparation of Escherichia coli cell extract for highly productive cell-free protein expression.
    J Struct Funct Genomics, Vol. 5, No. 1-2. (2004), pp. 63-68.
    posted to cell_free by viktor21614 on 2008-07-22 16:11:12 as ** along with 1 person Odi
  • Combination of cell-free expression and NMR spectroscopy as a new approach for structural investigation of membrane proteins.
    Magn Reson Chem, Vol. 44 Spec No (July 2006)
    posted to cell_free membrane_protein nmr by viktor21614 on 2008-07-24 08:50:26 as ** along with 1 person Odi
  • Prospects for lanthanides in structural biology by NMR
    Journal of Biomolecular NMR, Vol. 42, No. 1. (2008), pp. 1-9.
    by Gottfried Otting
    posted to rdc protein_ligand_interactions lanthanide cell_free by viktor21614 on 2008-09-15 17:22:29 as **
  • High efficiency cell-free synthesis of proteins: refinement of the coupled transcription/translation system.
    Anal Biochem, Vol. 206, No. 2. (1 November 1992), pp. 389-393.
    posted to cell_free by viktor21614 on 2008-07-22 15:51:36 as ** along with 1 person Odi
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