Soluble interleukin-6 receptors released from T cell or granulocyte/macrophage cell lines and human peripheral blood mononuclear cells are generated through an alternative splicing mechanism.

@article{citeulike:3043416, abstract = {To detect transcripts encoding the interleukin-6 receptor (IL-6R) molecule lacking the transmembrane (TM) domain, in various cell lines and peripheral blood mononuclear cells (PBMC), we used the polymerase chain reaction (PCR) with primer pairs that flank the TM domain and which were selected to generate a 398-bp fragment. We detected 398-bp and 304-bp DNA molecules in the PCR products of the U1, J22HL60, MT-2, MT-4, U937 and HL60 cell lines and of PBMC isolated from several individuals. The sequencing analysis of both DNA molecules showed that a 94-bp region consisting of the TM domain of IL-6R was deleted in the 304-bp molecule. Moreover, we detected a soluble (s) IL-6R protein of 45 kDa in culture supernatants of the MT-2, MT-4 and U937 cell lines by radioimmunoprecipitation using specific antibodies against sIL-6R. Our results indicate that active deletion of the TM domain by alternative splicing of mRNA represents one mechanism for release of sIL-6R into the culture supernatants of cells, or into serum or urine.}, address = {Department of Microbiology, Faculty of Medicine, Tokyo Medical and Dental University, Japan.}, author = {Horiuchi, S. and Koyanagi, Y. and Zhou, Y. and Miyamoto, H. and Tanaka, Y. and Waki, M. and Matsumoto, A. and Yamamoto, M. and Yamamoto, N. }, citeulike-article-id = {3043416}, issn = {0014-2980}, journal = {European journal of immunology}, keywords = {il6ra}, month = {August}, number = {8}, pages = {1945--1948}, posted-at = {2008-07-25 19:34:56}, priority = {2}, title = {Soluble interleukin-6 receptors released from T cell or granulocyte/macrophage cell lines and human peripheral blood mononuclear cells are generated through an alternative splicing mechanism.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/8056053}, volume = {24}, year = {1994} }

TY - JOUR ID - citeulike:3043416 L3 - citeulike-article-id:3043416 TI - Soluble interleukin-6 receptors released from T cell or granulocyte/macrophage cell lines and human peripheral blood mononuclear cells are generated through an alternative splicing mechanism. JF - European journal of immunology VL - 24 IS - 8 SP - 1945 EP - 1948 AD - Department of Microbiology, Faculty of Medicine, Tokyo Medical and Dental University, Japan. SN - 0014-2980 N2 - To detect transcripts encoding the interleukin-6 receptor (IL-6R) molecule lacking the transmembrane (TM) domain, in various cell lines and peripheral blood mononuclear cells (PBMC), we used the polymerase chain reaction (PCR) with primer pairs that flank the TM domain and which were selected to generate a 398-bp fragment. We detected 398-bp and 304-bp DNA molecules in the PCR products of the U1, J22HL60, MT-2, MT-4, U937 and HL60 cell lines and of PBMC isolated from several individuals. The sequencing analysis of both DNA molecules showed that a 94-bp region consisting of the TM domain of IL-6R was deleted in the 304-bp molecule. Moreover, we detected a soluble (s) IL-6R protein of 45 kDa in culture supernatants of the MT-2, MT-4 and U937 cell lines by radioimmunoprecipitation using specific antibodies against sIL-6R. Our results indicate that active deletion of the TM domain by alternative splicing of mRNA represents one mechanism for release of sIL-6R into the culture supernatants of cells, or into serum or urine. KW - il6ra AU - Horiuchi, S AU - Koyanagi, Y AU - Zhou, Y AU - Miyamoto, H AU - Tanaka, Y AU - Waki, M AU - Matsumoto, A AU - Yamamoto, M AU - Yamamoto, N PY - 1994/08// UR - http://view.ncbi.nlm.nih.gov/pubmed/8056053 ER -