Modification of Kolmogorov-Smirnov Test for DNA Content Data Analysis Through Distribution Alignment.

@article{citeulike:1903520, abstract = {The Kolmogorov-Smirnov (K-S) test is a statistical method often used for comparing two distributions. In high-throughput screening (HTS) studies, such distributions usually arise from the phenotype of independent cell populations. However, the K-S test has been criticized for being overly sensitive in applications, and it often detects a statistically significant difference that is not biologically meaningful. One major reason is that there is a common phenomenon in HTS studies that systematic drifting exists among the distributions due to reasons such as instrument variation, plate edge effect, accidental difference in sample handling, etc. In particular, in high-content cellular imaging experiments, the location shift could be dramatic since some compounds themselves are fluorescent. This oversensitivity of the K-S test is particularly overpowered in cellular assays where the sample sizes are very big (usually several thousands). In this paper, a modified K-S test is proposed to deal with the nonspecific location-shift problem in HTS studies. Specifically, we propose that the distributions are "normalized" by density curve alignment before the K-S test is conducted. In applications to simulation data and real experimental data, the results show that the proposed method has improved specificity.}, address = {Statistics and Information Science, Lilly Corporate Center, Indianapolis, IN.}, author = {Huang, Shuguang and Yeo, Adeline A A. and Li, Shuyu Dan D. }, citeulike-article-id = {1903520}, doi = {http://dx.doi.org/10.1089/adt.2007.071}, issn = {1540-658X}, journal = {Assay Drug Dev Technol}, keywords = {hcs, hts, method, statistics}, month = {October}, posted-at = {2007-11-12 17:27:55}, priority = {0}, title = {Modification of Kolmogorov-Smirnov Test for DNA Content Data Analysis Through Distribution Alignment.}, url = {http://dx.doi.org/10.1089/adt.2007.071}, year = {2007} }

TY - JOUR ID - citeulike:1903520 L3 - citeulike-article-id:1903520 N2 - The Kolmogorov-Smirnov (K-S) test is a statistical method often used for comparing two distributions. In high-throughput screening (HTS) studies, such distributions usually arise from the phenotype of independent cell populations. However, the K-S test has been criticized for being overly sensitive in applications, and it often detects a statistically significant difference that is not biologically meaningful. One major reason is that there is a common phenomenon in HTS studies that systematic drifting exists among the distributions due to reasons such as instrument variation, plate edge effect, accidental difference in sample handling, etc. In particular, in high-content cellular imaging experiments, the location shift could be dramatic since some compounds themselves are fluorescent. This oversensitivity of the K-S test is particularly overpowered in cellular assays where the sample sizes are very big (usually several thousands). In this paper, a modified K-S test is proposed to deal with the nonspecific location-shift problem in HTS studies. Specifically, we propose that the distributions are "normalized" by density curve alignment before the K-S test is conducted. In applications to simulation data and real experimental data, the results show that the proposed method has improved specificity. JF - Assay Drug Dev Technol SN - 1540-658X AD - Statistics and Information Science, Lilly Corporate Center, Indianapolis, IN. TI - Modification of Kolmogorov-Smirnov Test for DNA Content Data Analysis Through Distribution Alignment. KW - hcs KW - hts KW - method KW - statistics AU - Huang, Shuguang AU - Yeo, Adeline A AU - Li, Shuyu Dan PY - 2007/10/16/ UR - http://dx.doi.org/10.1089/adt.2007.071 ER -