Serotonin-dopamine interaction in the rat ventral tegmental area: an electrophysiological study in vivo.

@article{citeulike:2478869, abstract = {Electrophysiological techniques were used to study the effects of various serotonin (5-HT) agonists and antagonists on the activity of dopamine (DA) neurons in the ventral tegmental area (VTA) of rats. Systemic administration of the selective 5-HT1A receptor agonist 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT) (1.25-80 micrograms/kg i.v.) increased the firing rate of the majority (75\%) of DA cells studied and stimulated their bursting activity. A subpopulation (25\%) of DA neurons was inhibited by 8-OH-DPAT. Selective lesions of 5-HT neurons by the neurotoxin 5-7-dihydroxytryptamine abolished completely the excitatory effect of 8-OH-DPAT on both firing rate and bursting activity of DA neurons. Microiontophoretic application of 8-OH-DPAT into the VTA did not cause any change in the firing rate of DA neurons. Treatment with the selective 5-HT1B agonist CGS 12066B (7-trifluoromethyl-4-(4-methyl-1-piperazinyl)-pyrolo[1,2-a] quinoxaline 1:2 maleate salt) (1.25-160 micrograms/kg i.v.) did not cause any change in basal firing rate of VTA DA cells. Systemic administration of trifluoromethylphenylpiperazine (TFMPP) (1.25-160 micrograms/kg i.v.) and m-chlorophenylpiperazine (mCPP) (1.25-320 micrograms/kg i.v.), two mixed 5-HT1B/5-HT1C receptor agonists, significantly reduced the firing rate of all VTA DA neurons studied. The effect of mCPP (maximal inhibition, 40\%) was more pronounced compared to that of TFMPP (maximal inhibition, 25\%). Microiontophoretic application of mCPP into the VTA caused a marked inhibition of the basal activity of DA neurons.(ABSTRACT TRUNCATED AT 250 WORDS)}, address = {Istituto di Ricerche Farmacologiche Mario Negri, Chieti, Italy.}, author = {Prisco, S. and Pagannone, S. and Esposito, E. }, citeulike-article-id = {2478869}, issn = {0022-3565}, journal = {J Pharmacol Exp Ther}, keywords = {dopamine, electrophysiology, in-vivo, serotonin, vta}, month = {October}, number = {1}, pages = {83--90}, posted-at = {2008-03-06 14:02:31}, priority = {0}, title = {Serotonin-dopamine interaction in the rat ventral tegmental area: an electrophysiological study in vivo.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/7965760}, volume = {271}, year = {1994} }

TY - JOUR ID - citeulike:2478869 L3 - citeulike-article-id:2478869 TI - Serotonin-dopamine interaction in the rat ventral tegmental area: an electrophysiological study in vivo. JF - J Pharmacol Exp Ther VL - 271 IS - 1 SP - 83 EP - 90 AD - Istituto di Ricerche Farmacologiche Mario Negri, Chieti, Italy. SN - 0022-3565 N2 - Electrophysiological techniques were used to study the effects of various serotonin (5-HT) agonists and antagonists on the activity of dopamine (DA) neurons in the ventral tegmental area (VTA) of rats. Systemic administration of the selective 5-HT1A receptor agonist 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT) (1.25-80 micrograms/kg i.v.) increased the firing rate of the majority (75%) of DA cells studied and stimulated their bursting activity. A subpopulation (25%) of DA neurons was inhibited by 8-OH-DPAT. Selective lesions of 5-HT neurons by the neurotoxin 5-7-dihydroxytryptamine abolished completely the excitatory effect of 8-OH-DPAT on both firing rate and bursting activity of DA neurons. Microiontophoretic application of 8-OH-DPAT into the VTA did not cause any change in the firing rate of DA neurons. Treatment with the selective 5-HT1B agonist CGS 12066B (7-trifluoromethyl-4-(4-methyl-1-piperazinyl)-pyrolo[1,2-a] quinoxaline 1:2 maleate salt) (1.25-160 micrograms/kg i.v.) did not cause any change in basal firing rate of VTA DA cells. Systemic administration of trifluoromethylphenylpiperazine (TFMPP) (1.25-160 micrograms/kg i.v.) and m-chlorophenylpiperazine (mCPP) (1.25-320 micrograms/kg i.v.), two mixed 5-HT1B/5-HT1C receptor agonists, significantly reduced the firing rate of all VTA DA neurons studied. The effect of mCPP (maximal inhibition, 40%) was more pronounced compared to that of TFMPP (maximal inhibition, 25%). Microiontophoretic application of mCPP into the VTA caused a marked inhibition of the basal activity of DA neurons.(ABSTRACT TRUNCATED AT 250 WORDS) KW - dopamine KW - electrophysiology KW - in-vivo KW - serotonin KW - vta AU - Prisco, S AU - Pagannone, S AU - Esposito, E PY - 1994/10// UR - http://view.ncbi.nlm.nih.gov/pubmed/7965760 ER -