Cystic Fibrosis Sputum Supports Growth and Cues Key Aspects of Pseudomonas aeruginosa Physiology

by: Kelli L Palmer, Lauren M Mashburn, Pradeep K Singh, Marvin Whiteley

J. Bacteriol., Vol. 187, No. 15. (1 August 2005), pp. 5267-5277.

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Abstract

The opportunistic human pathogen Pseudomonas aeruginosa causes persistent airway infections in patients with cystic fibrosis (CF). To establish these chronic infections, P. aeruginosa must grow and proliferate within the highly viscous sputum in the lungs of CF patients. In this study, we used Affymetrix GeneChip microarrays to investigate the physiology of P. aeruginosa grown using CF sputum as the sole source of carbon and energy. Our results indicate that CF sputum readily supports high-density P. aeruginosa growth. Furthermore, multiple signals, which reduce swimming motility and prematurely activate the Pseudomonas quinolone signal cell-to-cell signaling cascade in P. aeruginosa, are present in CF sputum. P. aeruginosa factors critical for lysis of the common CF lung inhabitant Staphylococcus aureus were also induced in CF sputum and increased the competitiveness of P. aeruginosa during polymicrobial growth in CF sputum. 10.1128/JB.187.15.5267-5277.2005

@article{citeulike:2865626, abstract = {The opportunistic human pathogen Pseudomonas aeruginosa causes persistent airway infections in patients with cystic fibrosis (CF). To establish these chronic infections, P. aeruginosa must grow and proliferate within the highly viscous sputum in the lungs of CF patients. In this study, we used Affymetrix GeneChip microarrays to investigate the physiology of P. aeruginosa grown using CF sputum as the sole source of carbon and energy. Our results indicate that CF sputum readily supports high-density P. aeruginosa growth. Furthermore, multiple signals, which reduce swimming motility and prematurely activate the Pseudomonas quinolone signal cell-to-cell signaling cascade in P. aeruginosa, are present in CF sputum. P. aeruginosa factors critical for lysis of the common CF lung inhabitant Staphylococcus aureus were also induced in CF sputum and increased the competitiveness of P. aeruginosa during polymicrobial growth in CF sputum. 10.1128/JB.187.15.5267-5277.2005}, author = {Palmer, Kelli L. and Mashburn, Lauren M. and Singh, Pradeep K. and Whiteley, Marvin }, citeulike-article-id = {2865626}, doi = {http://dx.doi.org/10.1128/JB.187.15.5267-5277.2005}, journal = {J. Bacteriol.}, keywords = {cf, sputum}, month = {August}, number = {15}, pages = {5267--5277}, posted-at = {2008-06-05 15:02:46}, priority = {2}, title = {Cystic Fibrosis Sputum Supports Growth and Cues Key Aspects of Pseudomonas aeruginosa Physiology}, url = {http://dx.doi.org/10.1128/JB.187.15.5267-5277.2005}, volume = {187}, year = {2005} }

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TY - JOUR ID - citeulike:2865626 L3 - citeulike-article-id:2865626 TI - Cystic Fibrosis Sputum Supports Growth and Cues Key Aspects of Pseudomonas aeruginosa Physiology JF - J. Bacteriol. VL - 187 IS - 15 SP - 5267 EP - 5277 N2 - The opportunistic human pathogen Pseudomonas aeruginosa causes persistent airway infections in patients with cystic fibrosis (CF). To establish these chronic infections, P. aeruginosa must grow and proliferate within the highly viscous sputum in the lungs of CF patients. In this study, we used Affymetrix GeneChip microarrays to investigate the physiology of P. aeruginosa grown using CF sputum as the sole source of carbon and energy. Our results indicate that CF sputum readily supports high-density P. aeruginosa growth. Furthermore, multiple signals, which reduce swimming motility and prematurely activate the Pseudomonas quinolone signal cell-to-cell signaling cascade in P. aeruginosa, are present in CF sputum. P. aeruginosa factors critical for lysis of the common CF lung inhabitant Staphylococcus aureus were also induced in CF sputum and increased the competitiveness of P. aeruginosa during polymicrobial growth in CF sputum. 10.1128/JB.187.15.5267-5277.2005 KW - cf KW - sputum AU - Palmer, Kelli AU - Mashburn, Lauren AU - Singh, Pradeep AU - Whiteley, Marvin PY - 2005/08/01/ UR - http://dx.doi.org/10.1128/JB.187.15.5267-5277.2005 ER -

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