Immobilization of Escherichia coli expressing the lux genes of Xenorhabdus luminescens.

by: F Marincs, DW White

Appl. Environ. Microbiol., Vol. 60, No. 10. (1 October 1994), pp. 3862-3863.

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bioluminescence, plasmid

Abstract

The luxCDABE operon of Xenorhabdus luminescens was cloned into pUC18 to make pLITE27. Expression of the lux genes from the lac promoter resulted in strong constitutive light emission by Escherichia coli DH5 carrying the recombinant lux plasmid, pLITE27. When strain DH5(pLITE27) was immobilized with sodium alginate-CaCl2, the embedded cells retained their luminescence up to 2 weeks under appropriate storage conditions.

@article{citeulike:2933218, abstract = {The luxCDABE operon of Xenorhabdus luminescens was cloned into pUC18 to make pLITE27. Expression of the lux genes from the lac promoter resulted in strong constitutive light emission by Escherichia coli DH5 carrying the recombinant lux plasmid, pLITE27. When strain DH5(pLITE27) was immobilized with sodium alginate-CaCl2, the embedded cells retained their luminescence up to 2 weeks under appropriate storage conditions.}, author = {Marincs, F. and White, D. W. }, citeulike-article-id = {2933218}, journal = {Appl. Environ. Microbiol.}, keywords = {bioluminescence, plasmid}, month = {October}, number = {10}, pages = {3862--3863}, posted-at = {2008-06-27 11:12:26}, priority = {2}, title = {Immobilization of Escherichia coli expressing the lux genes of Xenorhabdus luminescens.}, url = {http://aem.asm.org/cgi/content/abstract/60/10/3862}, volume = {60}, year = {1994} }

RIS record

TY - JOUR ID - citeulike:2933218 L3 - citeulike-article-id:2933218 TI - Immobilization of Escherichia coli expressing the lux genes of Xenorhabdus luminescens. JF - Appl. Environ. Microbiol. VL - 60 IS - 10 SP - 3862 EP - 3863 N2 - The luxCDABE operon of Xenorhabdus luminescens was cloned into pUC18 to make pLITE27. Expression of the lux genes from the lac promoter resulted in strong constitutive light emission by Escherichia coli DH5 carrying the recombinant lux plasmid, pLITE27. When strain DH5(pLITE27) was immobilized with sodium alginate-CaCl2, the embedded cells retained their luminescence up to 2 weeks under appropriate storage conditions. KW - bioluminescence KW - plasmid AU - Marincs, F AU - White, DW PY - 1994/10/01/ UR - http://aem.asm.org/cgi/content/abstract/60/10/3862 ER -

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