PRG-1 and 21U-RNAs Interact to Form the piRNA Complex Required for Fertility in C. elegans

@article{citeulike:2948815, abstract = {Summary In metazoans, Piwi-related Argonaute proteins have been linked to germline maintenance, and to a class of germline-enriched small RNAs termed piRNAs. Here we show that an abundant class of 21 nucleotide small RNAs (21U-RNAs) are expressed in the C. elegans germline, interact with the C. elegans Piwi family member PRG-1, and depend on PRG-1 activity for their accumulation. The PRG-1 protein is expressed throughout development and localizes to nuage-like structures called P granules. Although 21U-RNA loci share a conserved upstream sequence motif, the mature 21U-RNAs are not conserved and, with few exceptions, fail to exhibit complementarity or evidence for direct regulation of other expressed sequences. Our findings demonstrate that 21U-RNAs are the piRNAs of C. elegans and link this class of small RNAs and their associated Piwi Argonaute to the maintenance of temperature-dependent fertility.}, author = {Batista, Pedro J. and Ruby, Graham J. and Claycomb, Julie M. and Chiang, Rosaria and Fahlgren, Noah and Kasschau, Kristin D. and Chaves, Daniel A. and Gu, Weifeng and Vasale, Jessica J. and Duan, Shenghua and Conte and Luo, Shujun and Schroth, Gary P. and Carrington, James C. and Bartel, David P. and Mello, Craig C. }, citeulike-article-id = {2948815}, doi = {http://dx.doi.org/10.1016/j.molcel.2008.06.002}, journal = {Molecular Cell}, keywords = {21u-rnas, fertility, piwi, prg-1}, posted-at = {2008-07-01 17:21:34}, priority = {5}, title = {PRG-1 and 21U-RNAs Interact to Form the piRNA Complex Required for Fertility in C. elegans}, url = {http://dx.doi.org/10.1016/j.molcel.2008.06.002}, volume = {In Press, Corrected Proof} }

TY - JOUR ID - citeulike:2948815 L3 - citeulike-article-id:2948815 TI - PRG-1 and 21U-RNAs Interact to Form the piRNA Complex Required for Fertility in C. elegans JF - Molecular Cell VL - In Press, Corrected Proof N2 - Summary In metazoans, Piwi-related Argonaute proteins have been linked to germline maintenance, and to a class of germline-enriched small RNAs termed piRNAs. Here we show that an abundant class of 21 nucleotide small RNAs (21U-RNAs) are expressed in the C. elegans germline, interact with the C. elegans Piwi family member PRG-1, and depend on PRG-1 activity for their accumulation. The PRG-1 protein is expressed throughout development and localizes to nuage-like structures called P granules. Although 21U-RNA loci share a conserved upstream sequence motif, the mature 21U-RNAs are not conserved and, with few exceptions, fail to exhibit complementarity or evidence for direct regulation of other expressed sequences. Our findings demonstrate that 21U-RNAs are the piRNAs of C. elegans and link this class of small RNAs and their associated Piwi Argonaute to the maintenance of temperature-dependent fertility. KW - 21u-rnas KW - fertility KW - piwi KW - prg-1 AU - Batista, Pedro AU - Ruby, Graham AU - Claycomb, Julie AU - Chiang, Rosaria AU - Fahlgren, Noah AU - Kasschau, Kristin AU - Chaves, Daniel AU - Gu, Weifeng AU - Vasale, Jessica AU - Duan, Shenghua AU - Conte AU - Luo, Shujun AU - Schroth, Gary AU - Carrington, James AU - Bartel, David AU - Mello, Craig UR - http://dx.doi.org/10.1016/j.molcel.2008.06.002 ER -