The regulatory activity of microRNA(*) species has substantial influence on microRNA and 3' UTR evolution.

@article{OKAMURA2008, abstract = {During microRNA (miRNA) biogenesis, one strand of a approximately 21-22-nucleotide RNA duplex is preferentially selected for entry into a silencing complex. The other strand, known as the miRNA(*) species, has typically been assumed to be a carrier strand. Here we show that, although Drosophila melanogaster miRNA(*) species are less abundant than their partners, they are often present at physiologically relevant levels and can associate with Argonaute proteins. Comparative genomic analyses revealed that >40\% of miRNA(*) sequences resist nucleotide divergence across Drosophilid evolution, and at least half of these well-conserved miRNA(*) species select for conserved 3' untranslated region seed matches well above background noise. Finally, we validated the inhibitory activity of miRNA(*) species in both cultured cells and transgenic animals. These data broaden the reach of the miRNA regulatory network and suggest an important mechanism that diversifies miRNA function during evolution.}, address = {Sloan-Kettering Institute, Department of Developmental Biology, 521 Rockefeller Research Laboratories, 1275 York Ave, Box 252, New York, New York 10065, USA.}, author = {Okamura, Katsutomo and Phillips, Michael D D. and Tyler, David M M. and Duan, Hong and Chou, Yu-Ting T. and Lai, Eric C C. }, citeulike-article-id = {2626565}, doi = {10.1038/nsmb.1409}, issn = {1545-9985}, journal = {Nature structural \& molecular biology}, keywords = {mirna}, month = {March}, posted-at = {2008-05-08 15:01:45}, priority = {2}, title = {The regulatory activity of microRNA(*) species has substantial influence on microRNA and 3' UTR evolution.}, url = {http://dx.doi.org/10.1038/nsmb.1409}, year = {2008} }

TY - JOUR ID - OKAMURA2008 L3 - citeulike-article-id:2626565 TI - The regulatory activity of microRNA(*) species has substantial influence on microRNA and 3' UTR evolution. JF - Nature structural & molecular biology AD - Sloan-Kettering Institute, Department of Developmental Biology, 521 Rockefeller Research Laboratories, 1275 York Ave, Box 252, New York, New York 10065, USA. SN - 1545-9985 N2 - During microRNA (miRNA) biogenesis, one strand of a approximately 21-22-nucleotide RNA duplex is preferentially selected for entry into a silencing complex. The other strand, known as the miRNA(*) species, has typically been assumed to be a carrier strand. Here we show that, although Drosophila melanogaster miRNA(*) species are less abundant than their partners, they are often present at physiologically relevant levels and can associate with Argonaute proteins. Comparative genomic analyses revealed that >40% of miRNA(*) sequences resist nucleotide divergence across Drosophilid evolution, and at least half of these well-conserved miRNA(*) species select for conserved 3' untranslated region seed matches well above background noise. Finally, we validated the inhibitory activity of miRNA(*) species in both cultured cells and transgenic animals. These data broaden the reach of the miRNA regulatory network and suggest an important mechanism that diversifies miRNA function during evolution. KW - mirna AU - Okamura, Katsutomo AU - Phillips, Michael D AU - Tyler, David M AU - Duan, Hong AU - Chou, Yu-Ting AU - Lai, Eric C PY - 2008/03/30/ UR - http://dx.doi.org/10.1038/nsmb.1409 ER -