Genome-wide analysis for discovery of rice microRNAs reveals natural antisense microRNAs (nat-miRNAs).

@article{LU2008, abstract = {Small RNAs (21-24 nt) are involved in gene regulation through translation inhibition, mRNA cleavage, or directing chromatin modifications. In rice, currently approximately 240 microRNAs (miRNAs) have been annotated. We sequenced more than four million small RNAs from rice and identified another 24 miRNA genes. Among these, we found a unique class of miRNAs that derive from natural cis-antisense transcript pairs. This configuration generates miRNAs that can perfectly match their targets. We provide evidence that the miRNAs function by inducing mRNA cleavage in the middle of their complementary site. Their production requires Dicer-like 1 (DCL1) activity, which is essential for canonical miRNA biogenesis. All of the natural antisense miRNAs (nat-miRNAs) identified in this study have large introns in their precursors that appear critical for nat-miRNA evolution and for the formation of functional miRNA loci. These findings suggest that other natural cis-antisense loci with similar exon-intron arrangements could be another source of miRNA genes.}, address = {Department of Plant and Soil Sciences and Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, USA.}, author = {Lu, C. and Jeong, D. H. and Kulkarni, K. and Pillay, M. and Nobuta, K. and German, R. and Thatcher, S. R. and Maher, C. and Zhang, L. and Ware, D. and Liu, B. and Cao, X. and Meyers, B. C. and Green, P. J. }, citeulike-article-id = {2771845}, doi = {http://dx.doi.org/10.1073/pnas.0708743105}, issn = {1091-6490}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {antisense, mirna}, month = {March}, number = {12}, pages = {4951--4956}, posted-at = {2008-05-08 15:18:04}, priority = {2}, title = {Genome-wide analysis for discovery of rice microRNAs reveals natural antisense microRNAs (nat-miRNAs).}, url = {http://dx.doi.org/10.1073/pnas.0708743105}, volume = {105}, year = {2008} }

TY - JOUR ID - LU2008 L3 - citeulike-article-id:2771845 TI - Genome-wide analysis for discovery of rice microRNAs reveals natural antisense microRNAs (nat-miRNAs). JF - Proceedings of the National Academy of Sciences of the United States of America VL - 105 IS - 12 SP - 4951 EP - 4956 AD - Department of Plant and Soil Sciences and Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, USA. SN - 1091-6490 N2 - Small RNAs (21-24 nt) are involved in gene regulation through translation inhibition, mRNA cleavage, or directing chromatin modifications. In rice, currently approximately 240 microRNAs (miRNAs) have been annotated. We sequenced more than four million small RNAs from rice and identified another 24 miRNA genes. Among these, we found a unique class of miRNAs that derive from natural cis-antisense transcript pairs. This configuration generates miRNAs that can perfectly match their targets. We provide evidence that the miRNAs function by inducing mRNA cleavage in the middle of their complementary site. Their production requires Dicer-like 1 (DCL1) activity, which is essential for canonical miRNA biogenesis. All of the natural antisense miRNAs (nat-miRNAs) identified in this study have large introns in their precursors that appear critical for nat-miRNA evolution and for the formation of functional miRNA loci. These findings suggest that other natural cis-antisense loci with similar exon-intron arrangements could be another source of miRNA genes. KW - antisense KW - mirna AU - Lu, C AU - Jeong, DH AU - Kulkarni, K AU - Pillay, M AU - Nobuta, K AU - German, R AU - Thatcher, SR AU - Maher, C AU - Zhang, L AU - Ware, D AU - Liu, B AU - Cao, X AU - Meyers, BC AU - Green, PJ PY - 2008/03/25/ UR - http://dx.doi.org/10.1073/pnas.0708743105 ER -