An Atomic Model of the Interferon-[beta] Enhanceosome

@article{citeulike:2961455, abstract = {Summary Transcriptional activation of the interferon-[beta] (IFN-[beta]) gene requires assembly of an enhanceosome containing ATF-2/c-Jun, IRF-3/IRF-7, and NF[kappa]B. These factors bind cooperatively to the IFN-[beta] enhancer and recruit coactivators and chromatin-remodeling proteins to the IFN-[beta] promoter. We describe here a crystal structure of the DNA-binding domains of IRF-3, IRF-7, and NF[kappa]B, bound to one half of the enhancer, and use a previously described structure of the remaining half to assemble a complete picture of enhanceosome architecture in the vicinity of the DNA. Association of eight proteins with the enhancer creates a continuous surface for recognizing a composite DNA-binding element. Paucity of local protein-protein contacts suggests that cooperative occupancy of the enhancer comes from both binding-induced changes in DNA conformation and interactions with additional components such as CBP. Contacts with virtually every nucleotide pair account for the evolutionary invariance of the enhancer sequence.}, author = {Panne, Daniel and Maniatis, Tom and Harrison, Stephen C. }, citeulike-article-id = {2961455}, doi = {http://dx.doi.org/10.1016/j.cell.2007.05.019}, journal = {Cell}, keywords = {cis\_regulatory\_evolution, dna-protein\_interaction, transcriptional\_regulation}, month = {June}, number = {6}, pages = {1111--1123}, posted-at = {2008-07-03 23:26:56}, priority = {2}, title = {An Atomic Model of the Interferon-[beta] Enhanceosome}, url = {http://dx.doi.org/10.1016/j.cell.2007.05.019}, volume = {129}, year = {2007} }

TY - JOUR ID - citeulike:2961455 L3 - citeulike-article-id:2961455 TI - An Atomic Model of the Interferon-[beta] Enhanceosome JF - Cell VL - 129 IS - 6 SP - 1111 EP - 1123 N2 - Summary Transcriptional activation of the interferon-[beta] (IFN-[beta]) gene requires assembly of an enhanceosome containing ATF-2/c-Jun, IRF-3/IRF-7, and NF[kappa]B. These factors bind cooperatively to the IFN-[beta] enhancer and recruit coactivators and chromatin-remodeling proteins to the IFN-[beta] promoter. We describe here a crystal structure of the DNA-binding domains of IRF-3, IRF-7, and NF[kappa]B, bound to one half of the enhancer, and use a previously described structure of the remaining half to assemble a complete picture of enhanceosome architecture in the vicinity of the DNA. Association of eight proteins with the enhancer creates a continuous surface for recognizing a composite DNA-binding element. Paucity of local protein-protein contacts suggests that cooperative occupancy of the enhancer comes from both binding-induced changes in DNA conformation and interactions with additional components such as CBP. Contacts with virtually every nucleotide pair account for the evolutionary invariance of the enhancer sequence. KW - cis_regulatory_evolution KW - dna-protein_interaction KW - transcriptional_regulation AU - Panne, Daniel AU - Maniatis, Tom AU - Harrison, Stephen PY - 2007/06/15/ UR - http://dx.doi.org/10.1016/j.cell.2007.05.019 ER -