A systematic bioinformatics approach for selection of epitope-based vaccine targets

@article{citeulike:1569195, abstract = {Epitope-based vaccines provide a new strategy for prophylactic and therapeutic application of pathogen-specific immunity. A critical requirement of this strategy is the identification and selection of T-cell epitopes that act as vaccine targets. This study describes current methodologies for the selection process, with dengue virus as a model system. A combination of publicly available bioinformatics algorithms and computational tools are used to screen and select antigen sequences as potential T-cell epitopes of supertype human leukocyte antigen (HLA) alleles. The selected sequences are tested for biological function by their activation of T-cells of HLA transgenic mice and of pathogen infected subjects. This approach provides an experimental basis for the design of pathogen specific, T-cell epitope-based vaccines that are targeted to majority of the genetic variants of the pathogen, and are effective for a broad range of differences in human leukocyte antigens among the global human population.}, author = {Khan, Asif M. and Miotto, Olivo and Heiny, A. T. and Salmon, Jerome and Srinivasan, K. N. and Nascimento, Eduardo J. and Marques and Brusic, Vladimir and Tan, Tin W. and August, Thomas J. }, booktitle = {International Conference on Immunogenomics and Immunomics, Budapest, Hungary, October 8-12, 2006}, citeulike-article-id = {1569195}, doi = {http://dx.doi.org/10.1016/j.cellimm.2007.02.005}, journal = {Cellular Immunology}, keywords = {immunoinformatics}, month = {December}, number = {2}, pages = {141--147}, posted-at = {2007-09-11 16:43:51}, priority = {5}, title = {A systematic bioinformatics approach for selection of epitope-based vaccine targets}, url = {http://dx.doi.org/10.1016/j.cellimm.2007.02.005}, volume = {244}, year = {2006} }

TY - JOUR ID - citeulike:1569195 L3 - citeulike-article-id:1569195 TI - A systematic bioinformatics approach for selection of epitope-based vaccine targets T2 - International Conference on Immunogenomics and Immunomics, Budapest, Hungary, October 8-12, 2006 JF - Cellular Immunology VL - 244 IS - 2 SP - 141 EP - 147 N2 - Epitope-based vaccines provide a new strategy for prophylactic and therapeutic application of pathogen-specific immunity. A critical requirement of this strategy is the identification and selection of T-cell epitopes that act as vaccine targets. This study describes current methodologies for the selection process, with dengue virus as a model system. A combination of publicly available bioinformatics algorithms and computational tools are used to screen and select antigen sequences as potential T-cell epitopes of supertype human leukocyte antigen (HLA) alleles. The selected sequences are tested for biological function by their activation of T-cells of HLA transgenic mice and of pathogen infected subjects. This approach provides an experimental basis for the design of pathogen specific, T-cell epitope-based vaccines that are targeted to majority of the genetic variants of the pathogen, and are effective for a broad range of differences in human leukocyte antigens among the global human population. KW - immunoinformatics AU - Khan, Asif AU - Miotto, Olivo AU - Heiny, AT AU - Salmon, Jerome AU - Srinivasan, KN AU - Nascimento, Eduardo AU - Marques AU - Brusic, Vladimir AU - Tan, Tin AU - August, Thomas PY - 2006/12// UR - http://dx.doi.org/10.1016/j.cellimm.2007.02.005 ER -