miR-125b inhibits osteoblastic differentiation by down-regulation of cell proliferation

@article{citeulike:2301470, abstract = {Although various microRNAs regulate cell differentiation and proliferation, no miRNA has been reported so far to play an important role in the regulation of osteoblast differentiation. Here we describe the role of miR-125b in osteoblastic differentiation in mouse mesenchymal stem cells, ST2, by regulating cell proliferation. The expression of miR-125b was time-dependently increased in ST2 cells, and the increase in miR-125b expression was attenuated in osteoblastic-differentiated ST2 cells induced by BMP-4. The transfection of exogenous miR-125b inhibited proliferation of ST2 cells and caused inhibition of osteoblastic differentiation. In contrast, when the endogenous miR-125b was blocked by transfection of its antisense RNA molecule, alkaline phosphatase activity after BMP-4 treatment was elevated. These results strongly suggest that miR-125b is involved in osteoblastic differentiation through the regulation of cell proliferation.}, author = {Mizuno, Yosuke and Yagi, Ken and Tokuzawa, Yoshimi and Kanesaki-Yatsuka, Yukiko and Suda, Tatsuo and Katagiri, Takenobu and Fukuda, Toru and Maruyama, Masayoshi and Okuda, Akihiko and Amemiya, Tomoyuki and Kondoh, Yasumitsu and Tashiro, Hideo and Okazaki, Yasushi }, citeulike-article-id = {2301470}, doi = {http://dx.doi.org/10.1016/j.bbrc.2008.01.073}, journal = {Biochemical and Biophysical Research Communications}, keywords = {mirna, osteoblast}, posted-at = {2008-01-29 09:45:47}, priority = {0}, title = {miR-125b inhibits osteoblastic differentiation by down-regulation of cell proliferation}, url = {http://dx.doi.org/10.1016/j.bbrc.2008.01.073}, volume = {In Press, Uncorrected Proof} }

TY - JOUR ID - citeulike:2301470 L3 - citeulike-article-id:2301470 TI - miR-125b inhibits osteoblastic differentiation by down-regulation of cell proliferation JF - Biochemical and Biophysical Research Communications VL - In Press, Uncorrected Proof N2 - Although various microRNAs regulate cell differentiation and proliferation, no miRNA has been reported so far to play an important role in the regulation of osteoblast differentiation. Here we describe the role of miR-125b in osteoblastic differentiation in mouse mesenchymal stem cells, ST2, by regulating cell proliferation. The expression of miR-125b was time-dependently increased in ST2 cells, and the increase in miR-125b expression was attenuated in osteoblastic-differentiated ST2 cells induced by BMP-4. The transfection of exogenous miR-125b inhibited proliferation of ST2 cells and caused inhibition of osteoblastic differentiation. In contrast, when the endogenous miR-125b was blocked by transfection of its antisense RNA molecule, alkaline phosphatase activity after BMP-4 treatment was elevated. These results strongly suggest that miR-125b is involved in osteoblastic differentiation through the regulation of cell proliferation. KW - mirna KW - osteoblast AU - Mizuno, Yosuke AU - Yagi, Ken AU - Tokuzawa, Yoshimi AU - Kanesaki-Yatsuka, Yukiko AU - Suda, Tatsuo AU - Katagiri, Takenobu AU - Fukuda, Toru AU - Maruyama, Masayoshi AU - Okuda, Akihiko AU - Amemiya, Tomoyuki AU - Kondoh, Yasumitsu AU - Tashiro, Hideo AU - Okazaki, Yasushi UR - http://dx.doi.org/10.1016/j.bbrc.2008.01.073 ER -