MicroRNA profiling of the murine hematopoietic system.

@article{citeulike:403156, abstract = {BACKGROUND: MicroRNAs (miRNAs) are a class of recently discovered noncoding RNA genes that post-transcriptionally regulate gene expression. It is becoming clear that miRNAs play an important role in the regulation of gene expression during development. However, in mammals, expression data are principally based on whole tissue analysis and are still very incomplete. RESULTS: We used oligonucleotide arrays to analyze miRNA expression in the murine hematopoietic system. Complementary oligonucleotides capable of hybridizing to 181 miRNAs were immobilized on a membrane and probed with radiolabeled RNA derived from low molecular weight fractions of total RNA from several different hematopoietic and neuronal cells. This method allowed us to analyze cell type-specific patterns of miRNA expression and to identify miRNAs that might be important for cell lineage specification and/or cell effector functions. CONCLUSION: This is the first report of systematic miRNA gene profiling in cells of the hematopoietic system. As expected, miRNA expression patterns were very different between hematopoietic and non-hematopoietic cells, with further subtle differences observed within the hematopoietic group. Interestingly, the most pronounced similarities were observed among fully differentiated effector cells (Th1 and Th2 lymphocytes and mast cells) and precursors at comparable stages of differentiation (double negative thymocytes and pro-B cells), suggesting that in addition to regulating the process of commitment to particular cellular lineages, miRNAs might have an important general role in the mechanism of cell differentiation and maintenance of cell identity.}, address = {Department of Pathology, Harvard Medical School, and CBR Institute for Biomedical Research, Boston, MA 02115, USA. monticel@cbr.med.harvard.edu}, author = {Monticelli, S. and Ansel, K. M. and Xiao, C. and Socci, N. D. and Krichevsky, A. M. and Thai, T. H. and Rajewsky, N. and Marks, D. S. and Sander, C. and Rajewsky, K. and Rao, A. and Kosik, K. S. }, citeulike-article-id = {403156}, doi = {http://dx.doi.org/10.1186/gb-2005-6-8-r71}, issn = {1465-6914}, journal = {Genome Biol}, keywords = {microrna, microrna\_expression}, number = {8}, posted-at = {2008-03-04 14:52:16}, priority = {2}, title = {MicroRNA profiling of the murine hematopoietic system.}, url = {http://dx.doi.org/10.1186/gb-2005-6-8-r71}, volume = {6}, year = {2005} }

TY - JOUR ID - citeulike:403156 L3 - citeulike-article-id:403156 TI - MicroRNA profiling of the murine hematopoietic system. JF - Genome Biol VL - 6 IS - 8 AD - Department of Pathology, Harvard Medical School, and CBR Institute for Biomedical Research, Boston, MA 02115, USA. monticel@cbr.med.harvard.edu SN - 1465-6914 N2 - BACKGROUND: MicroRNAs (miRNAs) are a class of recently discovered noncoding RNA genes that post-transcriptionally regulate gene expression. It is becoming clear that miRNAs play an important role in the regulation of gene expression during development. However, in mammals, expression data are principally based on whole tissue analysis and are still very incomplete. RESULTS: We used oligonucleotide arrays to analyze miRNA expression in the murine hematopoietic system. Complementary oligonucleotides capable of hybridizing to 181 miRNAs were immobilized on a membrane and probed with radiolabeled RNA derived from low molecular weight fractions of total RNA from several different hematopoietic and neuronal cells. This method allowed us to analyze cell type-specific patterns of miRNA expression and to identify miRNAs that might be important for cell lineage specification and/or cell effector functions. CONCLUSION: This is the first report of systematic miRNA gene profiling in cells of the hematopoietic system. As expected, miRNA expression patterns were very different between hematopoietic and non-hematopoietic cells, with further subtle differences observed within the hematopoietic group. Interestingly, the most pronounced similarities were observed among fully differentiated effector cells (Th1 and Th2 lymphocytes and mast cells) and precursors at comparable stages of differentiation (double negative thymocytes and pro-B cells), suggesting that in addition to regulating the process of commitment to particular cellular lineages, miRNAs might have an important general role in the mechanism of cell differentiation and maintenance of cell identity. KW - microrna KW - microrna_expression AU - Monticelli, S AU - Ansel, KM AU - Xiao, C AU - Socci, ND AU - Krichevsky, AM AU - Thai, TH AU - Rajewsky, N AU - Marks, DS AU - Sander, C AU - Rajewsky, K AU - Rao, A AU - Kosik, KS PY - 2005/// UR - http://dx.doi.org/10.1186/gb-2005-6-8-r71 ER -