Genomic Maps and Comparative Analysis of Histone Modifications in Human and Mouse

@article{citeulike:1899829, abstract = {We mapped histone H3 lysine 4 di- and trimethylation and lysine 9/14 acetylation across the nonrepetitive portions of human chromosomes 21 and 22 and compared patterns of lysine 4 dimethylation for several orthologous human and mouse loci. Both chromosomes show punctate sites enriched for modified histones. Sites showing trimethylation correlate with transcription starts, while those showing mainly dimethylation occur elsewhere in the vicinity of active genes. Punctate methylation patterns are also evident at the cytokine and IL-4 receptor loci. The Hox clusters present a strikingly different picture, with broad lysine 4-methylated regions that overlay multiple active genes. We suggest these regions represent active chromatin domains required for the maintenance of Hox gene expression. Methylation patterns at orthologous loci are strongly conserved between human and mouse even though many methylated sites do not show sequence conservation notably higher than background. This suggests that the DNA elements that direct the methylation represent only a small fraction of the region or lie at some distance from the site.}, author = {Bernstein, Bradley E. and Kamal, Michael and Lindblad-Toh, Kerstin and Bekiranov, Stefan and Bailey, Dione K. and Huebert, Dana J. and Mcmahon, Scott and Karlsson, Elinor K. and Kulbokas, Edward J. and Gingeras, Thomas R. and Schreiber, Stuart L. and Lander, Eric S. }, citeulike-article-id = {1899829}, comment = {Third, epigenome mapping in multiple species will add an evolutionary perspective to computational epigenetics. Initial results suggest that orthologous regions in different mammals carry similar epigenetic information (Bernstein et al., 2005; Enard et al., 2004), which is expected because the DNA encodes parts of its epigenetic state (Bock et al., 2007; Segal et al., 2006). It will be interesting to see whether comparative epigenomics can significantly improve our ability to identify functionally important sites in the human genome, as is the case for comparative genomics. - Bock et al 2007}, doi = {10.1016/j.cell.2005.01.001}, journal = {Cell}, keywords = {integration}, month = {January}, number = {2}, pages = {169--181}, posted-at = {2008-01-19 20:12:37}, priority = {3}, title = {Genomic Maps and Comparative Analysis of Histone Modifications in Human and Mouse}, url = {http://dx.doi.org/10.1016/j.cell.2005.01.001}, volume = {120}, year = {2005} }

TY - JOUR ID - citeulike:1899829 L3 - citeulike-article-id:1899829 TI - Genomic Maps and Comparative Analysis of Histone Modifications in Human and Mouse JF - Cell VL - 120 IS - 2 SP - 169 EP - 181 N2 - We mapped histone H3 lysine 4 di- and trimethylation and lysine 9/14 acetylation across the nonrepetitive portions of human chromosomes 21 and 22 and compared patterns of lysine 4 dimethylation for several orthologous human and mouse loci. Both chromosomes show punctate sites enriched for modified histones. Sites showing trimethylation correlate with transcription starts, while those showing mainly dimethylation occur elsewhere in the vicinity of active genes. Punctate methylation patterns are also evident at the cytokine and IL-4 receptor loci. The Hox clusters present a strikingly different picture, with broad lysine 4-methylated regions that overlay multiple active genes. We suggest these regions represent active chromatin domains required for the maintenance of Hox gene expression. Methylation patterns at orthologous loci are strongly conserved between human and mouse even though many methylated sites do not show sequence conservation notably higher than background. This suggests that the DNA elements that direct the methylation represent only a small fraction of the region or lie at some distance from the site. KW - integration N1 - Third, epigenome mapping in multiple species will add an evolutionary perspective to computational epigenetics. Initial results suggest that orthologous regions in different mammals carry similar epigenetic information (Bernstein et al., 2005; Enard et al., 2004), which is expected because the DNA encodes parts of its epigenetic state (Bock et al., 2007; Segal et al., 2006). It will be interesting to see whether comparative epigenomics can significantly improve our ability to identify functionally important sites in the human genome, as is the case for comparative genomics. - Bock et al 2007 AU - Bernstein, Bradley AU - Kamal, Michael AU - Lindblad-Toh, Kerstin AU - Bekiranov, Stefan AU - Bailey, Dione AU - Huebert, Dana AU - Mcmahon, Scott AU - Karlsson, Elinor AU - Kulbokas, Edward AU - Gingeras, Thomas AU - Schreiber, Stuart AU - Lander, Eric PY - 2005/01/28/ UR - http://dx.doi.org/10.1016/j.cell.2005.01.001 ER -