Targeted high-throughput sequencing of tagged nucleic acid samples

@article{citeulike:1537418, abstract = {High-throughput 454 DNA sequencing tech-nology allows much faster and more cost-effective sequencing than traditional Sanger sequencing. However, the technology imposes inherent limitations on the number of samples that can be processed in parallel. Here we introduce parallel tagged sequencing (PTS), a simple, inexpensive and flexible barcoding technique that can be used for parallel sequencing any number and type of double-stranded nucleic acid samples. We demonstrate that PTS is particularly powerful for sequencing contiguous DNA fragments such as mtDNA genomes: in theory as many as 250 mammalian mtDNA genomes can be sequenced in a single GS FLX run. PTS dramatically increases the sequencing throughput of samples in parallel and thus fully mobilizes the resources of the 454 technology for targeted sequencing. 10.1093/nar/gkm566}, author = {Meyer, Matthias and Stenzel, Udo and Myles, Sean and Prufer, Kay and Hofreiter, Michael }, citeulike-article-id = {1537418}, doi = {http://dx.doi.org/10.1093/nar/gkm566}, journal = {Nucl. Acids Res.}, month = {August}, pages = {gkm566+}, posted-at = {2007-08-06 05:17:36}, priority = {2}, title = {Targeted high-throughput sequencing of tagged nucleic acid samples}, url = {http://dx.doi.org/10.1093/nar/gkm566}, year = {2007} }

TY - JOUR ID - citeulike:1537418 L3 - citeulike-article-id:1537418 TI - Targeted high-throughput sequencing of tagged nucleic acid samples JF - Nucl. Acids Res. SP - gkm566 N2 - High-throughput 454 DNA sequencing tech-nology allows much faster and more cost-effective sequencing than traditional Sanger sequencing. However, the technology imposes inherent limitations on the number of samples that can be processed in parallel. Here we introduce parallel tagged sequencing (PTS), a simple, inexpensive and flexible barcoding technique that can be used for parallel sequencing any number and type of double-stranded nucleic acid samples. We demonstrate that PTS is particularly powerful for sequencing contiguous DNA fragments such as mtDNA genomes: in theory as many as 250 mammalian mtDNA genomes can be sequenced in a single GS FLX run. PTS dramatically increases the sequencing throughput of samples in parallel and thus fully mobilizes the resources of the 454 technology for targeted sequencing. 10.1093/nar/gkm566 AU - Meyer, Matthias AU - Stenzel, Udo AU - Myles, Sean AU - Prufer, Kay AU - Hofreiter, Michael PY - 2007/08/01/ UR - http://dx.doi.org/10.1093/nar/gkm566 ER -