PIASy-deficient mice display modest defects in IFN and Wnt signaling.

@article{citeulike:2690713, abstract = {Protein inhibitors of activated STATs (PIAS) represent a small family of nuclear proteins that modulate the activity of many transcription factors and act as E3 ligases for covalent modification of proteins with the small ubiquitin-like modifier (SUMO). In particular, PIASy has been shown to inhibit the activation of gene expression by the IFN-responsive transcription factor STAT1 and the Wnt-responsive transcription factor LEF1. To assess the function of PIASy in vivo, we generated and analyzed mice carrying a targeted mutation of the Piasy gene. We find that homozygous mutant mice have no obvious morphological defects and have a normal distribution of lymphocyte populations. Molecular analysis of signaling in response to IFN-gamma and Wnt agonists revealed a modest reduction in the activation of endogenous and transfected target genes. Two-dimensional analysis of total proteins and SUMO-modified proteins in transformed pre-B cells showed no significant differences between wild-type mice and homozygous mutant mice. Taken together, our data indicate that PIASy has a modest effect on cytokine and Wnt signaling, suggesting a redundancy with other members of the family of PIAS proteins.}, address = {Gene Center and Institute of Biochemistry, University of Munich, Munich, Germany.}, author = {Roth, W. and Sustmann, C. and Kieslinger, M. and Gilmozzi, A. and Irmer, D. and Kremmer, E. and Turck, C. and Grosschedl, R. }, citeulike-article-id = {2690713}, issn = {0022-1767}, journal = {Journal of immunology (Baltimore, Md. : 1950)}, month = {November}, number = {10}, pages = {6189--6199}, posted-at = {2008-04-19 15:34:03}, priority = {2}, title = {PIASy-deficient mice display modest defects in IFN and Wnt signaling.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/15528356}, volume = {173}, year = {2004} }

TY - JOUR ID - citeulike:2690713 L3 - citeulike-article-id:2690713 TI - PIASy-deficient mice display modest defects in IFN and Wnt signaling. JF - Journal of immunology (Baltimore, Md. : 1950) VL - 173 IS - 10 SP - 6189 EP - 6199 AD - Gene Center and Institute of Biochemistry, University of Munich, Munich, Germany. SN - 0022-1767 N2 - Protein inhibitors of activated STATs (PIAS) represent a small family of nuclear proteins that modulate the activity of many transcription factors and act as E3 ligases for covalent modification of proteins with the small ubiquitin-like modifier (SUMO). In particular, PIASy has been shown to inhibit the activation of gene expression by the IFN-responsive transcription factor STAT1 and the Wnt-responsive transcription factor LEF1. To assess the function of PIASy in vivo, we generated and analyzed mice carrying a targeted mutation of the Piasy gene. We find that homozygous mutant mice have no obvious morphological defects and have a normal distribution of lymphocyte populations. Molecular analysis of signaling in response to IFN-gamma and Wnt agonists revealed a modest reduction in the activation of endogenous and transfected target genes. Two-dimensional analysis of total proteins and SUMO-modified proteins in transformed pre-B cells showed no significant differences between wild-type mice and homozygous mutant mice. Taken together, our data indicate that PIASy has a modest effect on cytokine and Wnt signaling, suggesting a redundancy with other members of the family of PIAS proteins. AU - Roth, W AU - Sustmann, C AU - Kieslinger, M AU - Gilmozzi, A AU - Irmer, D AU - Kremmer, E AU - Turck, C AU - Grosschedl, R PY - 2004/11/15/ UR - http://view.ncbi.nlm.nih.gov/pubmed/15528356 ER -