Analysis of cell cycle phases and progression in cultured mammalian cells.

@article{citeulike:2784510, abstract = {Fluorescence activated cell sorting (FACS) analysis has become a standard tool to analyze cell cycle distributions in populations of cells. These methods require relatively large numbers of cells, and do not provide optimal resolution of the transitions between cell cycle phases. In this report we describe in detail complementary methods that utilize the incorporation of nucleotide analogs combined with microscopic examination. While often more time consuming, these protocols typically require far fewer cells, and allow accurate kinetic assessment of cell cycle progression. We also describe the use of a technique for the synchronization of adherent cells in mitosis by simple mechanical agitation (mitotic shake-off) that eliminates physiological perturbation associated with drug treatments.}, address = {Department of Molecular Biology, Cell Biology and Biochemistry, and Center for Genomics and Proteomics, Brown University, 70 Ship Street, Providence, RI 02903, USA.}, author = {Schorl, C. and Sedivy, J. M. }, citeulike-article-id = {2784510}, doi = {10.1016/j.ymeth.2006.07.022}, issn = {1046-2023}, journal = {Methods (San Diego, Calif.)}, month = {February}, number = {2}, pages = {143--150}, posted-at = {2008-05-11 15:47:06}, priority = {2}, title = {Analysis of cell cycle phases and progression in cultured mammalian cells.}, url = {http://dx.doi.org/10.1016/j.ymeth.2006.07.022}, volume = {41}, year = {2007} }

TY - JOUR ID - citeulike:2784510 TI - Analysis of cell cycle phases and progression in cultured mammalian cells. JF - Methods (San Diego, Calif.) VL - 41 IS - 2 SP - 143 EP - 150 AD - Department of Molecular Biology, Cell Biology and Biochemistry, and Center for Genomics and Proteomics, Brown University, 70 Ship Street, Providence, RI 02903, USA. SN - 1046-2023 N2 - Fluorescence activated cell sorting (FACS) analysis has become a standard tool to analyze cell cycle distributions in populations of cells. These methods require relatively large numbers of cells, and do not provide optimal resolution of the transitions between cell cycle phases. In this report we describe in detail complementary methods that utilize the incorporation of nucleotide analogs combined with microscopic examination. While often more time consuming, these protocols typically require far fewer cells, and allow accurate kinetic assessment of cell cycle progression. We also describe the use of a technique for the synchronization of adherent cells in mitosis by simple mechanical agitation (mitotic shake-off) that eliminates physiological perturbation associated with drug treatments. AU - Schorl, C AU - Sedivy, JM PY - 2007/02// UR - http://dx.doi.org/10.1016/j.ymeth.2006.07.022 ER -