Technical variables in high-throughput miRNA expression profiling: Much work remains to be done.

@article{citeulike:2997833, abstract = {MicroRNA (miRNA) gene expression profiling has provided important insights into plant and animal biology. However, there has not been ample published work about pitfalls associated with technical parameters in miRNA gene expression profiling. One source of pertinent information about technical variables in gene expression profiling is the separate and more well-established literature regarding mRNA expression profiling. However, many aspects of miRNA biochemistry are unique. For example, the cellular processing and compartmentation of miRNAs, the differential stability of specific miRNAs, and aspects of global miRNA expression regulation require specific consideration. Additional possible sources of systematic bias in miRNA expression studies include the differential impact of pre-analytical variables, substrate specificity of nucleic acid processing enzymes used in labeling and amplification, and issues regarding new miRNA discovery and annotation. We conclude that greater focus on technical parameters is required to bolster the validity, reliability, and cultural credibility of miRNA gene expression profiling studies.}, address = {Department of Pathology and Sanders-Brown Center, University of Kentucky, Lexington, KY 40536, USA.}, author = {Nelson, Peter T T. and Wang, Wang-Xia X. and Wilfred, Bernard R R. and Tang, Guiliang }, citeulike-article-id = {2997833}, doi = {http://dx.doi.org/10.1016/j.bbagrm.2008.03.012}, issn = {0006-3002}, journal = {Biochimica et biophysica acta}, keywords = {microarray, mirna}, month = {April}, posted-at = {2008-07-14 04:49:18}, priority = {2}, title = {Technical variables in high-throughput miRNA expression profiling: Much work remains to be done.}, url = {http://dx.doi.org/10.1016/j.bbagrm.2008.03.012}, year = {2008} }

TY - JOUR ID - citeulike:2997833 L3 - citeulike-article-id:2997833 TI - Technical variables in high-throughput miRNA expression profiling: Much work remains to be done. JF - Biochimica et biophysica acta AD - Department of Pathology and Sanders-Brown Center, University of Kentucky, Lexington, KY 40536, USA. SN - 0006-3002 N2 - MicroRNA (miRNA) gene expression profiling has provided important insights into plant and animal biology. However, there has not been ample published work about pitfalls associated with technical parameters in miRNA gene expression profiling. One source of pertinent information about technical variables in gene expression profiling is the separate and more well-established literature regarding mRNA expression profiling. However, many aspects of miRNA biochemistry are unique. For example, the cellular processing and compartmentation of miRNAs, the differential stability of specific miRNAs, and aspects of global miRNA expression regulation require specific consideration. Additional possible sources of systematic bias in miRNA expression studies include the differential impact of pre-analytical variables, substrate specificity of nucleic acid processing enzymes used in labeling and amplification, and issues regarding new miRNA discovery and annotation. We conclude that greater focus on technical parameters is required to bolster the validity, reliability, and cultural credibility of miRNA gene expression profiling studies. KW - microarray KW - mirna AU - Nelson, Peter T AU - Wang, Wang-Xia AU - Wilfred, Bernard R AU - Tang, Guiliang PY - 2008/04/07/ UR - http://dx.doi.org/10.1016/j.bbagrm.2008.03.012 ER -