Direct polymerase chain reaction (PCR) from human whole blood and filter-paper-dried blood by using a PCR buffer with a higher pH.

@article{citeulike:3013860, abstract = {We described a novel approach to directly amplify genomic DNA from whole blood and dried blood spotted on filter paper without any DNA isolation by using the PCR buffer with a higher pH, which was optimized as pH 9.1-9.6. Direct PCR on blood treated with various anticoagulants showed that the buffer worked well with the blood treated by citrate, EDTA, or heparinate. DNA fragments with different lengths could be efficiently amplified directly from various forms of blood samples. By coupling the buffer with tetra-PCR, a "true" single-tube genotyping was realized by using whole blood or paper-dried blood as starting material.}, address = {Huadong Research Institute for Medicine and Biotechnics, Nanjing, 210002, China.}, author = {Bu, Y. and Huang, H. and Zhou, G. }, citeulike-article-id = {3013860}, doi = {http://dx.doi.org/10.1016/j.ab.2008.01.010}, issn = {0003-2697}, journal = {Analytical biochemistry}, keywords = {blood, pcr}, month = {April}, number = {2}, pages = {370--372}, posted-at = {2008-07-17 09:37:15}, priority = {2}, title = {Direct polymerase chain reaction (PCR) from human whole blood and filter-paper-dried blood by using a PCR buffer with a higher pH.}, url = {http://dx.doi.org/10.1016/j.ab.2008.01.010}, volume = {375}, year = {2008} }

TY - JOUR ID - citeulike:3013860 L3 - citeulike-article-id:3013860 TI - Direct polymerase chain reaction (PCR) from human whole blood and filter-paper-dried blood by using a PCR buffer with a higher pH. JF - Analytical biochemistry VL - 375 IS - 2 SP - 370 EP - 372 AD - Huadong Research Institute for Medicine and Biotechnics, Nanjing, 210002, China. SN - 0003-2697 N2 - We described a novel approach to directly amplify genomic DNA from whole blood and dried blood spotted on filter paper without any DNA isolation by using the PCR buffer with a higher pH, which was optimized as pH 9.1-9.6. Direct PCR on blood treated with various anticoagulants showed that the buffer worked well with the blood treated by citrate, EDTA, or heparinate. DNA fragments with different lengths could be efficiently amplified directly from various forms of blood samples. By coupling the buffer with tetra-PCR, a "true" single-tube genotyping was realized by using whole blood or paper-dried blood as starting material. KW - blood KW - pcr AU - Bu, Y AU - Huang, H AU - Zhou, G PY - 2008/04/15/ UR - http://dx.doi.org/10.1016/j.ab.2008.01.010 ER -