Heparin is required for cell-free binding of basic fibroblast growth factor to a soluble receptor and for mitogenesis in whole cells.

@article{citeulike:2714267, abstract = {Heparin is required for the binding of basic fibroblast growth factor (bFGF) to high-affinity receptors on cells deficient in cell surface heparan sulfate proteoglycan. So that this heparin requirement could be evaluated in the absence of other cell surface molecules, we designed a simple assay based on a genetically engineered soluble form of murine FGF receptor 1 (mFR1) tagged with placental alkaline phosphatase. Using this assay, we showed that FGF-receptor binding has an absolute requirement for heparin. By using a cytokine-dependent lymphoid cell line engineered to express mFR1, we also showed that FGF-induced mitogenic activity is heparin dependent. Furthermore, we tested a series of small heparin oligosaccharides of defined lengths for their abilities to support bFGF-receptor binding and biologic activity. We found that a heparin oligosaccharide with as few as eight sugar residues is sufficient to support these activities. We also demonstrated that heparin facilitates FGF dimerization, a property that may be important for receptor activation.}, address = {Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115.}, author = {Ornitz, D. M. and Yayon, A. and Flanagan, J. G. and Svahn, C. M. and Levi, E. and Leder, P. }, citeulike-article-id = {2714267}, issn = {0270-7306}, journal = {Molecular and cellular biology}, keywords = {fgf}, month = {January}, number = {1}, pages = {240--247}, posted-at = {2008-04-24 21:12:04}, priority = {2}, title = {Heparin is required for cell-free binding of basic fibroblast growth factor to a soluble receptor and for mitogenesis in whole cells.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/1309590}, volume = {12}, year = {1992} }

TY - JOUR ID - citeulike:2714267 L3 - citeulike-article-id:2714267 TI - Heparin is required for cell-free binding of basic fibroblast growth factor to a soluble receptor and for mitogenesis in whole cells. JF - Molecular and cellular biology VL - 12 IS - 1 SP - 240 EP - 247 AD - Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115. SN - 0270-7306 N2 - Heparin is required for the binding of basic fibroblast growth factor (bFGF) to high-affinity receptors on cells deficient in cell surface heparan sulfate proteoglycan. So that this heparin requirement could be evaluated in the absence of other cell surface molecules, we designed a simple assay based on a genetically engineered soluble form of murine FGF receptor 1 (mFR1) tagged with placental alkaline phosphatase. Using this assay, we showed that FGF-receptor binding has an absolute requirement for heparin. By using a cytokine-dependent lymphoid cell line engineered to express mFR1, we also showed that FGF-induced mitogenic activity is heparin dependent. Furthermore, we tested a series of small heparin oligosaccharides of defined lengths for their abilities to support bFGF-receptor binding and biologic activity. We found that a heparin oligosaccharide with as few as eight sugar residues is sufficient to support these activities. We also demonstrated that heparin facilitates FGF dimerization, a property that may be important for receptor activation. KW - fgf AU - Ornitz, DM AU - Yayon, A AU - Flanagan, JG AU - Svahn, CM AU - Levi, E AU - Leder, P PY - 1992/01// UR - http://view.ncbi.nlm.nih.gov/pubmed/1309590 ER -