The Chemical Genomic Portrait of Yeast: Uncovering a Phenotype for All Genes

by: Maureen E Hillenmeyer, Eula Fung, Jan Wildenhain, Sarah E Pierce, Shawn Hoon, William Lee, Michael Proctor, St, Mike Tyers, Daphne Koller, Russ B Altman, Ronald W Davis, Corey Nislow, Guri Giaever

Science, Vol. 320, No. 5874. (18 April 2008), pp. 362-365.

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Abstract

Genetics aims to understand the relation between genotype and phenotype. However, because complete deletion of most yeast genes ([~]80%) has no obvious phenotypic consequence in rich medium, it is difficult to study their functions. To uncover phenotypes for this nonessential fraction of the genome, we performed 1144 chemical genomic assays on the yeast whole-genome heterozygous and homozygous deletion collections and quantified the growth fitness of each deletion strain in the presence of chemical or environmental stress conditions. We found that 97% of gene deletions exhibited a measurable growth phenotype, suggesting that nearly all genes are essential for optimal growth in at least one condition. 10.1126/science.1150021

@article{citeulike:2687203, abstract = {Genetics aims to understand the relation between genotype and phenotype. However, because complete deletion of most yeast genes ([~]80\%) has no obvious phenotypic consequence in rich medium, it is difficult to study their functions. To uncover phenotypes for this nonessential fraction of the genome, we performed 1144 chemical genomic assays on the yeast whole-genome heterozygous and homozygous deletion collections and quantified the growth fitness of each deletion strain in the presence of chemical or environmental stress conditions. We found that 97\% of gene deletions exhibited a measurable growth phenotype, suggesting that nearly all genes are essential for optimal growth in at least one condition. 10.1126/science.1150021}, author = {Hillenmeyer, Maureen E. and Fung, Eula and Wildenhain, Jan and Pierce, Sarah E. and Hoon, Shawn and Lee, William and Proctor, Michael and St and Tyers, Mike and Koller, Daphne and Altman, Russ B. and Davis, Ronald W. and Nislow, Corey and Giaever, Guri }, citeulike-article-id = {2687203}, doi = {10.1126/science.1150021}, journal = {Science}, keywords = {phenotype, yeast}, month = {April}, number = {5874}, pages = {362--365}, posted-at = {2008-05-12 13:08:52}, priority = {2}, title = {The Chemical Genomic Portrait of Yeast: Uncovering a Phenotype for All Genes}, url = {http://dx.doi.org/10.1126/science.1150021}, volume = {320}, year = {2008} }

RIS record

TY - JOUR ID - citeulike:2687203 L3 - citeulike-article-id:2687203 TI - The Chemical Genomic Portrait of Yeast: Uncovering a Phenotype for All Genes JF - Science VL - 320 IS - 5874 SP - 362 EP - 365 N2 - Genetics aims to understand the relation between genotype and phenotype. However, because complete deletion of most yeast genes ([~]80%) has no obvious phenotypic consequence in rich medium, it is difficult to study their functions. To uncover phenotypes for this nonessential fraction of the genome, we performed 1144 chemical genomic assays on the yeast whole-genome heterozygous and homozygous deletion collections and quantified the growth fitness of each deletion strain in the presence of chemical or environmental stress conditions. We found that 97% of gene deletions exhibited a measurable growth phenotype, suggesting that nearly all genes are essential for optimal growth in at least one condition. 10.1126/science.1150021 KW - phenotype KW - yeast AU - Hillenmeyer, Maureen AU - Fung, Eula AU - Wildenhain, Jan AU - Pierce, Sarah AU - Hoon, Shawn AU - Lee, William AU - Proctor, Michael AU - St AU - Tyers, Mike AU - Koller, Daphne AU - Altman, Russ AU - Davis, Ronald AU - Nislow, Corey AU - Giaever, Guri PY - 2008/04/18/ UR - http://dx.doi.org/10.1126/science.1150021 ER -

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