A Novel Gene of HIV-1, vpu, and its 16-Kilodalton Product

@article{citeulike:1531395, abstract = {A 16-kilodalton protein expressed in cells producing the human immunodeficiency virus (HIV-1) was identified as the gene product of the vpu open reading frame. When expressed in vitro, the 81-amino acid vpu protein reacted with about one-third of the serum samples from AIDS patients that were tested, indicating that the vpu open reading frame is expressed in vivo as well. Introduction of a frame-shift mutation into the vpu open reading frame did not significantly interfere with expression of the major viral proteins in a transient expression system. However, a five- to tenfold reduction in progeny virions was observed after the infection of T lymphocytes with the mutant virus. These data suggest that the vpu gene product is required for efficient virus replication and may have a role in assembly or maturation of progeny virions.}, author = {Strebel, Klaus and Klimkait, Thomas and Martin, Malcolm A. }, citeulike-article-id = {1531395}, keywords = {binding, gene, hiv, ic, new, site}, posted-at = {2007-08-02 23:16:36}, priority = {5}, title = {A Novel Gene of HIV-1, vpu, and its 16-Kilodalton Product}, url = {http://www.jstor.org/stable/1702727} }

TY - JOUR ID - citeulike:1531395 L3 - citeulike-article-id:1531395 TI - A Novel Gene of HIV-1, vpu, and its 16-Kilodalton Product N2 - A 16-kilodalton protein expressed in cells producing the human immunodeficiency virus (HIV-1) was identified as the gene product of the vpu open reading frame. When expressed in vitro, the 81-amino acid vpu protein reacted with about one-third of the serum samples from AIDS patients that were tested, indicating that the vpu open reading frame is expressed in vivo as well. Introduction of a frame-shift mutation into the vpu open reading frame did not significantly interfere with expression of the major viral proteins in a transient expression system. However, a five- to tenfold reduction in progeny virions was observed after the infection of T lymphocytes with the mutant virus. These data suggest that the vpu gene product is required for efficient virus replication and may have a role in assembly or maturation of progeny virions. KW - binding KW - gene KW - hiv KW - ic KW - new KW - site AU - Strebel, Klaus AU - Klimkait, Thomas AU - Martin, Malcolm UR - http://www.jstor.org/stable/1702727 ER -