Global organization of metabolic fluxes in the bacterium Escherichia coli.

@article{citeulike:363627, abstract = {Cellular metabolism, the integrated interconversion of thousands of metabolic substrates through enzyme-catalysed biochemical reactions, is the most investigated complex intracellular web of molecular interactions. Although the topological organization of individual reactions into metabolic networks is well understood, the principles that govern their global functional use under different growth conditions raise many unanswered questions. By implementing a flux balance analysis of the metabolism of Escherichia coli strain MG1655, here we show that network use is highly uneven. Whereas most metabolic reactions have low fluxes, the overall activity of the metabolism is dominated by several reactions with very high fluxes. E. coli responds to changes in growth conditions by reorganizing the rates of selected fluxes predominantly within this high-flux backbone. This behaviour probably represents a universal feature of metabolic activity in all cells, with potential implications for metabolic engineering.}, address = {Department of Physics, University of Notre Dame, Notre Dame, Indiana 46556, USA.}, author = {Almaas, E. and Kov\'{a}cs, B. and Vicsek, T. and Oltvai, Z. N. and Barab\'{a}si, A. L. }, citeulike-article-id = {363627}, doi = {http://dx.doi.org/10.1038/nature02289}, issn = {1476-4687}, journal = {Nature}, keywords = {ecoli, flux-balance-analysis, metabolism}, month = {February}, number = {6977}, pages = {839--843}, posted-at = {2008-06-16 10:50:39}, priority = {4}, title = {Global organization of metabolic fluxes in the bacterium Escherichia coli.}, url = {http://dx.doi.org/10.1038/nature02289}, volume = {427}, year = {2004} }

TY - JOUR ID - citeulike:363627 L3 - citeulike-article-id:363627 TI - Global organization of metabolic fluxes in the bacterium Escherichia coli. JF - Nature VL - 427 IS - 6977 SP - 839 EP - 843 AD - Department of Physics, University of Notre Dame, Notre Dame, Indiana 46556, USA. SN - 1476-4687 N2 - Cellular metabolism, the integrated interconversion of thousands of metabolic substrates through enzyme-catalysed biochemical reactions, is the most investigated complex intracellular web of molecular interactions. Although the topological organization of individual reactions into metabolic networks is well understood, the principles that govern their global functional use under different growth conditions raise many unanswered questions. By implementing a flux balance analysis of the metabolism of Escherichia coli strain MG1655, here we show that network use is highly uneven. Whereas most metabolic reactions have low fluxes, the overall activity of the metabolism is dominated by several reactions with very high fluxes. E. coli responds to changes in growth conditions by reorganizing the rates of selected fluxes predominantly within this high-flux backbone. This behaviour probably represents a universal feature of metabolic activity in all cells, with potential implications for metabolic engineering. KW - ecoli KW - flux-balance-analysis KW - metabolism AU - Almaas, E AU - Kovács, B AU - Vicsek, T AU - Oltvai, ZN AU - Barabási, AL PY - 2004/02/26/ UR - http://dx.doi.org/10.1038/nature02289 ER -