Towards defining the nuclear proteome

@article{citeulike:2278653, abstract = {BACKGROUND:The nucleus is a complex cellular organelle and accurately defining its protein content is essential before any systematic characterisation can be considered. RESULTS:Here, we report direct evidence for 2568 mammalian proteins within the nuclear proteome: the nuclear subcellular localization of 1529 proteins based on a high-throughput subcellular localization protocol of full-length proteins and an additional 1039 proteins for which clear experimental evidence is documented in published literature. CONCLUSIONS:This represents direct experimental evidence that the nuclear proteome consists of at least 14\% of the entire proteome. This high-quality nuclear proteome dataset was used to evaluate computational approaches designed to identify additional nuclear proteins. Based on this analysis researchers can determine the stringency and types of lines of evidence they consider to infer the size and complement of the nuclear proteome.}, author = {Fink, Lynn J. and Karunaratne, Seetha and Mittal, Amit and Gardiner, Donald and Hamilton, Nicholas and Mahony, Donna and Kai, Chikatoshi and Suzuki, Harukazu and Hayashizaki, Yosihide and Teasdale, Rohan }, citeulike-article-id = {2278653}, doi = {10.1186/gb-2008-9-1-r15}, journal = {Genome Biology}, keywords = {bioinformatics, localisation, nls, nuclear, proteome, scl}, number = {1}, posted-at = {2008-01-23 02:50:10}, priority = {2}, title = {Towards defining the nuclear proteome}, url = {http://dx.doi.org/10.1186/gb-2008-9-1-r15}, volume = {9}, year = {2008} }

TY - JOUR ID - citeulike:2278653 L3 - citeulike-article-id:2278653 TI - Towards defining the nuclear proteome JF - Genome Biology VL - 9 IS - 1 N2 - BACKGROUND:The nucleus is a complex cellular organelle and accurately defining its protein content is essential before any systematic characterisation can be considered. RESULTS:Here, we report direct evidence for 2568 mammalian proteins within the nuclear proteome: the nuclear subcellular localization of 1529 proteins based on a high-throughput subcellular localization protocol of full-length proteins and an additional 1039 proteins for which clear experimental evidence is documented in published literature. CONCLUSIONS:This represents direct experimental evidence that the nuclear proteome consists of at least 14% of the entire proteome. This high-quality nuclear proteome dataset was used to evaluate computational approaches designed to identify additional nuclear proteins. Based on this analysis researchers can determine the stringency and types of lines of evidence they consider to infer the size and complement of the nuclear proteome. KW - bioinformatics KW - localisation KW - nls KW - nuclear KW - proteome KW - scl AU - Fink, Lynn AU - Karunaratne, Seetha AU - Mittal, Amit AU - Gardiner, Donald AU - Hamilton, Nicholas AU - Mahony, Donna AU - Kai, Chikatoshi AU - Suzuki, Harukazu AU - Hayashizaki, Yosihide AU - Teasdale, Rohan PY - 2008/// UR - http://dx.doi.org/10.1186/gb-2008-9-1-r15 ER -