Advances in the Analysis of Protein Phosphorylation

@article{citeulike:2498286, abstract = {Abstract: Phosphorylation is one of the most relevant and ubiquitous post-translational modifications. Despite its relevance, the analysis of protein phosphorylation has been revealed as one of the most challenging tasks due to its highly dynamic nature and low stoichiometry. However, the development and introduction of new analytical methods are modifying rapidly and substantially this field. Especially important has been the introduction of more sensitive and specific methods for phosphoprotein and phosphopeptide purification as well as the use of more sensitive and accurate MS-based analytical methods. The integration of both approaches has enabled large-scale phosphoproteome studies to be performed, an unimaginable task few years ago. Additionally, methods originally developed for differential proteomics have been adapted making the study of the highly dynamic nature of protein phosphorylation feasible. This review aims at offering an overview on the most frequently used methods in phosphoprotein and phosphopeptide enrichment as well as on the most recent MS-based analysis strategies. Current strategies for quantitative phosphoproteomics and the study of the dynamics of protein phosphorylation are highlighted.}, address = {Departamento de Prote\'{o}mica, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cient\'{i}ficas, c/ Darwin 3, 28049 Madrid, Spain}, author = {Paradela, Alberto and Albar, Juan P. }, citeulike-article-id = {2498286}, doi = {10.1021/pr7006544}, journal = {J. Proteome Res.}, keywords = {analysis, methods, phosphorylation, proteomics, review}, month = {March}, posted-at = {2008-03-10 02:28:00}, priority = {2}, title = {Advances in the Analysis of Protein Phosphorylation}, url = {http://dx.doi.org/10.1021/pr7006544}, year = {2008} }

TY - JOUR ID - citeulike:2498286 L3 - citeulike-article-id:2498286 TI - Advances in the Analysis of Protein Phosphorylation JF - J. Proteome Res. AD - Departamento de Proteómica, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Científicas, c/ Darwin 3, 28049 Madrid, Spain N2 - Abstract: Phosphorylation is one of the most relevant and ubiquitous post-translational modifications. Despite its relevance, the analysis of protein phosphorylation has been revealed as one of the most challenging tasks due to its highly dynamic nature and low stoichiometry. However, the development and introduction of new analytical methods are modifying rapidly and substantially this field. Especially important has been the introduction of more sensitive and specific methods for phosphoprotein and phosphopeptide purification as well as the use of more sensitive and accurate MS-based analytical methods. The integration of both approaches has enabled large-scale phosphoproteome studies to be performed, an unimaginable task few years ago. Additionally, methods originally developed for differential proteomics have been adapted making the study of the highly dynamic nature of protein phosphorylation feasible. This review aims at offering an overview on the most frequently used methods in phosphoprotein and phosphopeptide enrichment as well as on the most recent MS-based analysis strategies. Current strategies for quantitative phosphoproteomics and the study of the dynamics of protein phosphorylation are highlighted. KW - analysis KW - methods KW - phosphorylation KW - proteomics KW - review AU - Paradela, Alberto AU - Albar, Juan PY - 2008/03/08/ UR - http://dx.doi.org/10.1021/pr7006544 ER -