Cold adaptation of the Antarctic archaeon, Methanococcoides burtonii assessed by proteomics using ICAT.

@article{citeulike:2783954, abstract = {Using isotope coded affinity tag (ICAT) chromatography and liquid chromatography-mass spectrometry, 163 proteins were identified from the cold-adapted archaeon, Methanococcoides burtonii. 14 proteins were differentially expressed during growth at 4 degrees C and 23 degrees C. Knowledge of protein abundance, protein identity and gene arrangement was used to determine mechanisms of cold adaptation. Growth temperature was found to affect proteins involved in energy generation and biosynthesis linked to methanogenesis, membrane transport, transcription and protein folding, as well as affecting the expression of two hypothetical proteins. Pooling the data from this ICAT study with data from a previous two-dimensional gel electrophoresis study highlighted consistencies and differences between the two methods, and led us to conclude that the two approaches were generally complementary. This is the first report of ICAT applied to Archaea, or for the study of cold adaptation in any organism.}, address = {School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, 2052, NSW, Australia.}, author = {Goodchild, A. and Raftery, M. and Saunders, N. F. and Guilhaus, M. and Cavicchioli, R. }, citeulike-article-id = {2783954}, doi = {10.1021/pr049760p}, issn = {1535-3893}, journal = {Journal of proteome research}, keywords = {antarctic, archaea, icat, methanococcoides, proteomics, psychrophily}, number = {2}, pages = {473--480}, posted-at = {2008-05-11 10:08:25}, priority = {2}, title = {Cold adaptation of the Antarctic archaeon, Methanococcoides burtonii assessed by proteomics using ICAT.}, url = {http://dx.doi.org/10.1021/pr049760p}, volume = {4}, year = {2005} }

TY - JOUR ID - citeulike:2783954 L3 - citeulike-article-id:2783954 TI - Cold adaptation of the Antarctic archaeon, Methanococcoides burtonii assessed by proteomics using ICAT. JF - Journal of proteome research VL - 4 IS - 2 SP - 473 EP - 480 AD - School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, 2052, NSW, Australia. SN - 1535-3893 N2 - Using isotope coded affinity tag (ICAT) chromatography and liquid chromatography-mass spectrometry, 163 proteins were identified from the cold-adapted archaeon, Methanococcoides burtonii. 14 proteins were differentially expressed during growth at 4 degrees C and 23 degrees C. Knowledge of protein abundance, protein identity and gene arrangement was used to determine mechanisms of cold adaptation. Growth temperature was found to affect proteins involved in energy generation and biosynthesis linked to methanogenesis, membrane transport, transcription and protein folding, as well as affecting the expression of two hypothetical proteins. Pooling the data from this ICAT study with data from a previous two-dimensional gel electrophoresis study highlighted consistencies and differences between the two methods, and led us to conclude that the two approaches were generally complementary. This is the first report of ICAT applied to Archaea, or for the study of cold adaptation in any organism. KW - antarctic KW - archaea KW - icat KW - methanococcoides KW - proteomics KW - psychrophily AU - Goodchild, A AU - Raftery, M AU - Saunders, NF AU - Guilhaus, M AU - Cavicchioli, R PY - 2005///r UR - http://dx.doi.org/10.1021/pr049760p ER -