A generic protein purification method for protein complex characterization and proteome exploration.

by: G Rigaut, A Shevchenko, B Rutz, M Wilm, M Mann, B Séraphin

Nat Biotechnol, Vol. 17, No. 10. (October 1999), pp. 1030-1032.

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Abstract

We have developed a generic procedure to purify proteins expressed at their natural level under native conditions using a novel tandem affinity purification (TAP) tag. The TAP tag allows the rapid purification of complexes from a relatively small number of cells without prior knowledge of the complex composition, activity, or function. Combined with mass spectrometry, the TAP strategy allows for the identification of proteins interacting with a given target protein. The TAP method has been tested in yeast but should be applicable to other cells or organisms.

@article{citeulike:100011, abstract = {We have developed a generic procedure to purify proteins expressed at their natural level under native conditions using a novel tandem affinity purification (TAP) tag. The TAP tag allows the rapid purification of complexes from a relatively small number of cells without prior knowledge of the complex composition, activity, or function. Combined with mass spectrometry, the TAP strategy allows for the identification of proteins interacting with a given target protein. The TAP method has been tested in yeast but should be applicable to other cells or organisms.}, address = {European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany.}, author = {Rigaut, G. and Shevchenko, A. and Rutz, B. and Wilm, M. and Mann, M. and S\'{e}raphin, B. }, citeulike-article-id = {100011}, doi = {http://dx.doi.org/10.1038/13732}, issn = {1087-0156}, journal = {Nat Biotechnol}, keywords = {immunoprecipitation}, month = {October}, number = {10}, pages = {1030--1032}, posted-at = {2007-03-22 23:15:44}, priority = {3}, title = {A generic protein purification method for protein complex characterization and proteome exploration.}, url = {http://dx.doi.org/10.1038/13732}, volume = {17}, year = {1999} }

RIS record

TY - JOUR ID - citeulike:100011 L3 - citeulike-article-id:100011 TI - A generic protein purification method for protein complex characterization and proteome exploration. JF - Nat Biotechnol VL - 17 IS - 10 SP - 1030 EP - 1032 AD - European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany. SN - 1087-0156 N2 - We have developed a generic procedure to purify proteins expressed at their natural level under native conditions using a novel tandem affinity purification (TAP) tag. The TAP tag allows the rapid purification of complexes from a relatively small number of cells without prior knowledge of the complex composition, activity, or function. Combined with mass spectrometry, the TAP strategy allows for the identification of proteins interacting with a given target protein. The TAP method has been tested in yeast but should be applicable to other cells or organisms. KW - immunoprecipitation AU - Rigaut, G AU - Shevchenko, A AU - Rutz, B AU - Wilm, M AU - Mann, M AU - Séraphin, B PY - 1999/10// UR - http://dx.doi.org/10.1038/13732 ER -

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