Src Tyrosine Kinase Is a Novel Direct Effector of G Proteins

@article{citeulike:708167, abstract = {Heterotrimeric G proteins transduce signals from cell surface receptors to modulate the activity of cellular effectors. Src, the product of the first characterized proto-oncogene and the first identified protein tyrosine kinase, plays a critical role in the signal transduction of G protein-coupled receptors. However, the mechanism of biochemical regulation of Src by G proteins is not known. Here we demonstrate that G[alpha]s and G[alpha]i, but neither G[alpha]q, G[alpha]12 nor G[beta][gamma], directly stimulate the kinase activity of downregulated c-Src. G[alpha]s and G[alpha]i similarly modulate Hck, another member of Src-family tyrosine kinases. G[alpha]s and G[alpha]i bind to the catalytic domain and change the conformation of Src, leading to increased accessibility of the active site to substrates. These data demonstrate that the Src family tyrosine kinases are direct effectors of G proteins.}, author = {Ma, Yong-Chao and Huang, Jianyun and Ali, Shariq and Lowry, William and Huang, Xin-Yun }, citeulike-article-id = {708167}, doi = {10.1016/S0092-8674(00)00086-6}, journal = {Cell}, keywords = {cxcr4, tcrmr}, month = {September}, number = {5}, pages = {635--646}, posted-at = {2006-06-23 09:43:07}, priority = {2}, title = {Src Tyrosine Kinase Is a Novel Direct Effector of G Proteins}, url = {http://dx.doi.org/10.1016/S0092-8674(00)00086-6}, volume = {102}, year = {2000} }

TY - JOUR ID - citeulike:708167 L3 - citeulike-article-id:708167 TI - Src Tyrosine Kinase Is a Novel Direct Effector of G Proteins JF - Cell VL - 102 IS - 5 SP - 635 EP - 646 N2 - Heterotrimeric G proteins transduce signals from cell surface receptors to modulate the activity of cellular effectors. Src, the product of the first characterized proto-oncogene and the first identified protein tyrosine kinase, plays a critical role in the signal transduction of G protein-coupled receptors. However, the mechanism of biochemical regulation of Src by G proteins is not known. Here we demonstrate that G[alpha]s and G[alpha]i, but neither G[alpha]q, G[alpha]12 nor G[beta][gamma], directly stimulate the kinase activity of downregulated c-Src. G[alpha]s and G[alpha]i similarly modulate Hck, another member of Src-family tyrosine kinases. G[alpha]s and G[alpha]i bind to the catalytic domain and change the conformation of Src, leading to increased accessibility of the active site to substrates. These data demonstrate that the Src family tyrosine kinases are direct effectors of G proteins. KW - cxcr4 KW - tcrmr AU - Ma, Yong-Chao AU - Huang, Jianyun AU - Ali, Shariq AU - Lowry, William AU - Huang, Xin-Yun PY - 2000/09/01/ UR - http://dx.doi.org/10.1016/S0092-8674(00)00086-6 ER -