A single Hox locus in Drosophila produces functional microRNAs from opposite DNA strands

by: Alexander Stark, Natascha Bushati, Calvin H Jan, Pouya Kheradpour, Emily Hodges, Julius Brennecke, David P Bartel, Stephen M Cohen, Manolis Kellis

Genes Dev., Vol. 22, No. 1. (1 January 2008), pp. 8-13.

View FullText article

DOI, HighWire, Pubmed, Hubmed

Reviews [Write a review of this article]

There are no reviews of this article

Find related articles from these CiteULike users

sebastien_vigneau, stajich, heliopais, Berkeley Mycology (group), Alexander_Kanitz, maralena

Find related articles with these CiteULike tags

antisense, comparative_genomics, drosophila, expression, functional, hox, microrna, mirna, noncoding_rna, sense, transcription

Abstract

MicroRNAs (miRNAs) are [~]22-nucleotide RNAs that are processed from characteristic precursor hairpins and pair to sites in messages of protein-coding genes to direct post-transcriptional repression. Here, we report that the miRNA iab-4 locus in the Drosophila Hox cluster is transcribed convergently from both DNA strands, giving rise to two distinct functional miRNAs. Both sense and antisense miRNA products target neighboring Hox genes via highly conserved sites, leading to homeotic transformations when ectopically expressed. We also report sense/antisense miRNAs in mouse and find antisense transcripts close to many miRNAs in both flies and mammals, suggesting that additional sense/antisense pairs exist. 10.1101/gad.1613108

@article{citeulike:2197060, abstract = {MicroRNAs (miRNAs) are [\~{}]22-nucleotide RNAs that are processed from characteristic precursor hairpins and pair to sites in messages of protein-coding genes to direct post-transcriptional repression. Here, we report that the miRNA iab-4 locus in the Drosophila Hox cluster is transcribed convergently from both DNA strands, giving rise to two distinct functional miRNAs. Both sense and antisense miRNA products target neighboring Hox genes via highly conserved sites, leading to homeotic transformations when ectopically expressed. We also report sense/antisense miRNAs in mouse and find antisense transcripts close to many miRNAs in both flies and mammals, suggesting that additional sense/antisense pairs exist. 10.1101/gad.1613108}, author = {Stark, Alexander and Bushati, Natascha and Jan, Calvin H. and Kheradpour, Pouya and Hodges, Emily and Brennecke, Julius and Bartel, David P. and Cohen, Stephen M. and Kellis, Manolis }, citeulike-article-id = {2197060}, doi = {http://dx.doi.org/10.1101/gad.1613108}, journal = {Genes Dev.}, keywords = {comparative\_genomics, drosophila, mirna, noncoding\_rna}, month = {January}, number = {1}, pages = {8--13}, posted-at = {2008-04-05 19:55:44}, priority = {2}, title = {A single Hox locus in Drosophila produces functional microRNAs from opposite DNA strands}, url = {http://dx.doi.org/10.1101/gad.1613108}, volume = {22}, year = {2008} }

RIS record

TY - JOUR ID - citeulike:2197060 L3 - citeulike-article-id:2197060 TI - A single Hox locus in Drosophila produces functional microRNAs from opposite DNA strands JF - Genes Dev. VL - 22 IS - 1 SP - 8 EP - 13 N2 - MicroRNAs (miRNAs) are [~]22-nucleotide RNAs that are processed from characteristic precursor hairpins and pair to sites in messages of protein-coding genes to direct post-transcriptional repression. Here, we report that the miRNA iab-4 locus in the Drosophila Hox cluster is transcribed convergently from both DNA strands, giving rise to two distinct functional miRNAs. Both sense and antisense miRNA products target neighboring Hox genes via highly conserved sites, leading to homeotic transformations when ectopically expressed. We also report sense/antisense miRNAs in mouse and find antisense transcripts close to many miRNAs in both flies and mammals, suggesting that additional sense/antisense pairs exist. 10.1101/gad.1613108 KW - comparative_genomics KW - drosophila KW - mirna KW - noncoding_rna AU - Stark, Alexander AU - Bushati, Natascha AU - Jan, Calvin AU - Kheradpour, Pouya AU - Hodges, Emily AU - Brennecke, Julius AU - Bartel, David AU - Cohen, Stephen AU - Kellis, Manolis PY - 2008/01/01/ UR - http://dx.doi.org/10.1101/gad.1613108 ER -

RIS BibTeX