Detection of Low-Copy-Number Genomic DNA Sequences in Individual Bacterial Cells by Using Peptide Nucleic Acid-Assisted Rolling-Circle Amplification and Fluorescence In Situ Hybridization

by: Irina Smolina, Charles Lee, Maxim Frank-Kamenetskii

Appl. Environ. Microbiol., Vol. 73, No. 7. (1 April 2007), pp. 2324-2328.

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Abstract

An approach is proposed for in situ detection of short signature DNA sequences present in single copies per bacterial genome. The site is locally opened by peptide nucleic acids, and a circular oligonucleotide is assembled. The amplicon generated by rolling circle amplification is detected by hybridization with fluorescently labeled decorator probes. 10.1128/AEM.02038-06

@article{citeulike:2749919, abstract = {An approach is proposed for in situ detection of short signature DNA sequences present in single copies per bacterial genome. The site is locally opened by peptide nucleic acids, and a circular oligonucleotide is assembled. The amplicon generated by rolling circle amplification is detected by hybridization with fluorescently labeled decorator probes. 10.1128/AEM.02038-06}, author = {Smolina, Irina and Lee, Charles and Frank-Kamenetskii, Maxim }, citeulike-article-id = {2749919}, doi = {10.1128/AEM.02038-06}, journal = {Appl. Environ. Microbiol.}, keywords = {fish, rca}, month = {April}, number = {7}, pages = {2324--2328}, posted-at = {2008-05-03 20:25:43}, priority = {2}, title = {Detection of Low-Copy-Number Genomic DNA Sequences in Individual Bacterial Cells by Using Peptide Nucleic Acid-Assisted Rolling-Circle Amplification and Fluorescence In Situ Hybridization}, url = {http://dx.doi.org/10.1128/AEM.02038-06}, volume = {73}, year = {2007} }

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TY - JOUR ID - citeulike:2749919 L3 - citeulike-article-id:2749919 TI - Detection of Low-Copy-Number Genomic DNA Sequences in Individual Bacterial Cells by Using Peptide Nucleic Acid-Assisted Rolling-Circle Amplification and Fluorescence In Situ Hybridization JF - Appl. Environ. Microbiol. VL - 73 IS - 7 SP - 2324 EP - 2328 N2 - An approach is proposed for in situ detection of short signature DNA sequences present in single copies per bacterial genome. The site is locally opened by peptide nucleic acids, and a circular oligonucleotide is assembled. The amplicon generated by rolling circle amplification is detected by hybridization with fluorescently labeled decorator probes. 10.1128/AEM.02038-06 KW - fish KW - rca AU - Smolina, Irina AU - Lee, Charles AU - Frank-Kamenetskii, Maxim PY - 2007/04/01/ UR - http://dx.doi.org/10.1128/AEM.02038-06 ER -

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