Quantitative Imaging of Nitrogen Fixation by Individual Bacteria Within Animal Cells

by: Claude P Lechene, Yvette Luyten, Gregory Mcmahon, Daniel L Distel

Science, Vol. 317, No. 5844. (14 September 2007), pp. 1563-1566.

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Abstract

Biological nitrogen fixation, the conversion of atmospheric nitrogen to ammonia for biosynthesis, is exclusively performed by a few bacteria and archaea. Despite the essential importance of biological nitrogen fixation, it has been impossible to quantify the incorporation of nitrogen by individual bacteria or to map the fate of fixed nitrogen in host cells. In this study, with multi-isotope imaging mass spectrometry we directly imaged and measured nitrogen fixation by individual bacteria within eukaryotic host cells and demonstrated that fixed nitrogen is used for host metabolism. This approach introduces a powerful way to study microbes and global nutrient cycles. 10.1126/science.1145557

@article{citeulike:2750766, abstract = {Biological nitrogen fixation, the conversion of atmospheric nitrogen to ammonia for biosynthesis, is exclusively performed by a few bacteria and archaea. Despite the essential importance of biological nitrogen fixation, it has been impossible to quantify the incorporation of nitrogen by individual bacteria or to map the fate of fixed nitrogen in host cells. In this study, with multi-isotope imaging mass spectrometry we directly imaged and measured nitrogen fixation by individual bacteria within eukaryotic host cells and demonstrated that fixed nitrogen is used for host metabolism. This approach introduces a powerful way to study microbes and global nutrient cycles. 10.1126/science.1145557}, author = {Lechene, Claude P. and Luyten, Yvette and Mcmahon, Gregory and Distel, Daniel L. }, citeulike-article-id = {2750766}, doi = {10.1126/science.1145557}, journal = {Science}, keywords = {fish, mar-fish}, month = {September}, number = {5844}, pages = {1563--1566}, posted-at = {2008-05-03 22:40:01}, priority = {2}, title = {Quantitative Imaging of Nitrogen Fixation by Individual Bacteria Within Animal Cells}, url = {http://dx.doi.org/10.1126/science.1145557}, volume = {317}, year = {2007} }

RIS record

TY - JOUR ID - citeulike:2750766 L3 - citeulike-article-id:2750766 TI - Quantitative Imaging of Nitrogen Fixation by Individual Bacteria Within Animal Cells JF - Science VL - 317 IS - 5844 SP - 1563 EP - 1566 N2 - Biological nitrogen fixation, the conversion of atmospheric nitrogen to ammonia for biosynthesis, is exclusively performed by a few bacteria and archaea. Despite the essential importance of biological nitrogen fixation, it has been impossible to quantify the incorporation of nitrogen by individual bacteria or to map the fate of fixed nitrogen in host cells. In this study, with multi-isotope imaging mass spectrometry we directly imaged and measured nitrogen fixation by individual bacteria within eukaryotic host cells and demonstrated that fixed nitrogen is used for host metabolism. This approach introduces a powerful way to study microbes and global nutrient cycles. 10.1126/science.1145557 KW - fish KW - mar-fish AU - Lechene, Claude AU - Luyten, Yvette AU - Mcmahon, Gregory AU - Distel, Daniel PY - 2007/09/14/ UR - http://dx.doi.org/10.1126/science.1145557 ER -

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