Usefulness of length heterogeneity-PCR for monitoring lactic acid bacteria succession during maize ensiling

@article{citeulike:2758420, abstract = {Abstract The use of length-heterogeneity PCR was explored to monitor lactic acid bacteria succession during ensiling of maize. Bacterial diversity was studied during the fermentation of 30-day-old maize in optimal and spoilage-simulating conditions. A length heterogeneity PCR profile database of lactic acid bacteria isolated from the silage and identified by 16S rRNA gene sequencing was established. Although interoperonic 16S rRNA gene length polymorphisms were detected in some isolates, strain analysis showed that most of the lactic acid bacteria species thriving in silage could be discriminated by this method. The length heterogeneity PCR profiles of bacterial communities during maize fermentation were compared with those on a database. Under optimal fermentation conditions all the ecological indices of bacterial diversity, richness and evenness, deduced from community profiles, increased until day thirteen of fermentation and then decreased to the initial values. Pediococcus and Weissella dominated, especially in the first days of fermentation. Lactococcus lactis ssp. lactis and Lactobacillus brevis were mainly found after six days of fermentation. A peak corresponding to Lactobacillus plantarum was present in all the fermentation phases, but was only a minor fraction of the population. Unsuitable fermentation conditions and withered maize leaves in the presence of oxygen and water excess caused an enrichment of Enterococcus sp. and Enterobacter sp.}, author = {Brusetti, Lorenzo and Borin, Sara and Mora, Diego and Rizzi, Aurora and Raddadi, Noura and Sorlini, Claudia and Daffonchio, Daniele }, citeulike-article-id = {2758420}, doi = {10.1111/j.1574-6941.2005.00059.x}, journal = {FEMS Microbiology Ecology}, keywords = {lh-pcr}, number = {1}, pages = {154--164}, posted-at = {2008-05-05 18:07:04}, priority = {2}, title = {Usefulness of length heterogeneity-PCR for monitoring lactic acid bacteria succession during maize ensiling}, url = {http://www.blackwell-synergy.com/doi/abs/10.1111/j.1574-6941.2005.00059.x}, volume = {56}, year = {2006} }

TY - JOUR ID - citeulike:2758420 L3 - citeulike-article-id:2758420 TI - Usefulness of length heterogeneity-PCR for monitoring lactic acid bacteria succession during maize ensiling JF - FEMS Microbiology Ecology VL - 56 IS - 1 SP - 154 EP - 164 N2 - Abstract The use of length-heterogeneity PCR was explored to monitor lactic acid bacteria succession during ensiling of maize. Bacterial diversity was studied during the fermentation of 30-day-old maize in optimal and spoilage-simulating conditions. A length heterogeneity PCR profile database of lactic acid bacteria isolated from the silage and identified by 16S rRNA gene sequencing was established. Although interoperonic 16S rRNA gene length polymorphisms were detected in some isolates, strain analysis showed that most of the lactic acid bacteria species thriving in silage could be discriminated by this method. The length heterogeneity PCR profiles of bacterial communities during maize fermentation were compared with those on a database. Under optimal fermentation conditions all the ecological indices of bacterial diversity, richness and evenness, deduced from community profiles, increased until day thirteen of fermentation and then decreased to the initial values. Pediococcus and Weissella dominated, especially in the first days of fermentation. Lactococcus lactis ssp. lactis and Lactobacillus brevis were mainly found after six days of fermentation. A peak corresponding to Lactobacillus plantarum was present in all the fermentation phases, but was only a minor fraction of the population. Unsuitable fermentation conditions and withered maize leaves in the presence of oxygen and water excess caused an enrichment of Enterococcus sp. and Enterobacter sp. KW - lh-pcr AU - Brusetti, Lorenzo AU - Borin, Sara AU - Mora, Diego AU - Rizzi, Aurora AU - Raddadi, Noura AU - Sorlini, Claudia AU - Daffonchio, Daniele PY - 2006/// UR - http://www.blackwell-synergy.com/doi/abs/10.1111/j.1574-6941.2005.00059.x ER -