Biosynthesis of 2-methylbacteriohopanepolyols by an anoxygenic phototroph

@article{citeulike:2785943, abstract = {Sedimentary 2-methyhopanes have been used as biomarker proxies for cyanobacteria, the only known bacterial clade capable of oxygenic photosynthesis and the only group of organisms found thus far to produce abundant 2-methylbacteriohopanepolyols (2-MeBHPs). Here, we report the identification of significant quantities of 2-MeBHP in two strains of the anoxygenic phototroph Rhodopseudomonas palustris. Biosynthesis of 2-MeBHP can occur in the absence of O2, deriving the C-2 methyl group from methionine. The relative abundance of 2-MeBHP varies considerably with culture conditions, ranging from 13.3\% of total bacteriohopanepolyol (BHP) to trace levels of methylation. Analysis of intact BHPs reveals the presence of methylated bacteriohopane-32,33,34,35-tetrol but no detectable methylation of 35-aminobacteriohopane-32,33,34-triol. Our results demonstrate that an anoxygenic photoautotroph is capable of generating 2-MeBHPs and show that the potential origins of sedimentary 2-methylhopanoids are more diverse than previously thought. 10.1073/pnas.0704912104}, author = {Rashby, Sky E. and Sessions, Alex L. and Summons, Roger E. and Newman, Dianne K. }, citeulike-article-id = {2785943}, doi = {10.1073/pnas.0704912104}, journal = {Proceedings of the National Academy of Sciences}, keywords = {lipids}, month = {September}, number = {38}, pages = {15099--15104}, posted-at = {2008-05-12 00:09:08}, priority = {2}, title = {Biosynthesis of 2-methylbacteriohopanepolyols by an anoxygenic phototroph}, url = {http://dx.doi.org/10.1073/pnas.0704912104}, volume = {104}, year = {2007} }

TY - JOUR ID - citeulike:2785943 L3 - citeulike-article-id:2785943 TI - Biosynthesis of 2-methylbacteriohopanepolyols by an anoxygenic phototroph JF - Proceedings of the National Academy of Sciences VL - 104 IS - 38 SP - 15099 EP - 15104 N2 - Sedimentary 2-methyhopanes have been used as biomarker proxies for cyanobacteria, the only known bacterial clade capable of oxygenic photosynthesis and the only group of organisms found thus far to produce abundant 2-methylbacteriohopanepolyols (2-MeBHPs). Here, we report the identification of significant quantities of 2-MeBHP in two strains of the anoxygenic phototroph Rhodopseudomonas palustris. Biosynthesis of 2-MeBHP can occur in the absence of O2, deriving the C-2 methyl group from methionine. The relative abundance of 2-MeBHP varies considerably with culture conditions, ranging from 13.3% of total bacteriohopanepolyol (BHP) to trace levels of methylation. Analysis of intact BHPs reveals the presence of methylated bacteriohopane-32,33,34,35-tetrol but no detectable methylation of 35-aminobacteriohopane-32,33,34-triol. Our results demonstrate that an anoxygenic photoautotroph is capable of generating 2-MeBHPs and show that the potential origins of sedimentary 2-methylhopanoids are more diverse than previously thought. 10.1073/pnas.0704912104 KW - lipids AU - Rashby, Sky AU - Sessions, Alex AU - Summons, Roger AU - Newman, Dianne PY - 2007/09/18/ UR - http://dx.doi.org/10.1073/pnas.0704912104 ER -