Direct profiling of environmental microbial populations by thermal dissociation analysis of native rRNAs hybridized to oligonucleotide microarrays.

by: S El Fantroussi, H Urakawa, AE Bernhard, JJ Kelly, PA Noble, H Smidt, GM Yershov, DA Stahl

Applied and environmental microbiology, Vol. 69, No. 4. (April 2003), pp. 2377-2382.

View FullText article

DOI, HighWire, Pubmed, Hubmed

Reviews [Write a review of this article]

There are no reviews of this article

Find related articles from these CiteULike users

vrich, mcox

Find related articles with these CiteULike tags

16s, diversity, microarray, thermal_dissociation

Abstract

Oligonucleotide microarrays were used to profile directly extracted rRNA from environmental microbial populations without PCR amplification. In our initial inspection of two distinct estuarine study sites, the hybridization patterns were reproducible and varied between estuarine sediments of differing salinities. The determination of a thermal dissociation curve (i.e., melting profile) for each probe-target duplex provided information on hybridization specificity, which is essential for confirming adequate discrimination between target and nontarget sequences.

@article{citeulike:2790013, abstract = {Oligonucleotide microarrays were used to profile directly extracted rRNA from environmental microbial populations without PCR amplification. In our initial inspection of two distinct estuarine study sites, the hybridization patterns were reproducible and varied between estuarine sediments of differing salinities. The determination of a thermal dissociation curve (i.e., melting profile) for each probe-target duplex provided information on hybridization specificity, which is essential for confirming adequate discrimination between target and nontarget sequences.}, address = {Department of Civil and Environmental Engineering, University of Washington, Seattle 98195, USA.}, author = {El Fantroussi, S. and Urakawa, H. and Bernhard, A. E. and Kelly, J. J. and Noble, P. A. and Smidt, H. and Yershov, G. M. and Stahl, D. A. }, citeulike-article-id = {2790013}, doi = {10.1128/AEM.69.4.2377-2382.2003}, issn = {0099-2240}, journal = {Applied and environmental microbiology}, keywords = {microarray, thermal\_dissociation}, month = {April}, number = {4}, pages = {2377--2382}, posted-at = {2008-05-16 02:06:10}, priority = {2}, title = {Direct profiling of environmental microbial populations by thermal dissociation analysis of native rRNAs hybridized to oligonucleotide microarrays.}, url = {http://dx.doi.org/10.1128/AEM.69.4.2377-2382.2003}, volume = {69}, year = {2003} }

RIS record

TY - JOUR ID - citeulike:2790013 L3 - citeulike-article-id:2790013 TI - Direct profiling of environmental microbial populations by thermal dissociation analysis of native rRNAs hybridized to oligonucleotide microarrays. JF - Applied and environmental microbiology VL - 69 IS - 4 SP - 2377 EP - 2382 AD - Department of Civil and Environmental Engineering, University of Washington, Seattle 98195, USA. SN - 0099-2240 N2 - Oligonucleotide microarrays were used to profile directly extracted rRNA from environmental microbial populations without PCR amplification. In our initial inspection of two distinct estuarine study sites, the hybridization patterns were reproducible and varied between estuarine sediments of differing salinities. The determination of a thermal dissociation curve (i.e., melting profile) for each probe-target duplex provided information on hybridization specificity, which is essential for confirming adequate discrimination between target and nontarget sequences. KW - microarray KW - thermal_dissociation AU - El Fantroussi, S AU - Urakawa, H AU - Bernhard, AE AU - Kelly, JJ AU - Noble, PA AU - Smidt, H AU - Yershov, GM AU - Stahl, DA PY - 2003/04// UR - http://dx.doi.org/10.1128/AEM.69.4.2377-2382.2003 ER -

RIS BibTeX