The different structures containing tight junction proteins in epidermal and other stratified epithelial cells, including squamous cell metaplasia

@article{citeulike:2943643, abstract = {In stratified squamous epithelia constituent proteins of tight junctions (TJs) are not restricted to the zonula occludens-related structures of the uppermost living cell layer such as the stratum granulosum of the epidermis but TJ membrane proteins such as occludin and certain members of the claudin family as well as TJ plaque proteins, notably cingulin and protein ZO-1, have also been identified by immunofluorescence and immunoelectron microscopy in more basal layers where they form special cell-cell-connecting structures such as the "lamellated" and the "sandwich" junctions. In the present study, we describe another TJ protein-containing structure, the very small puncta occludentia ("stud junctions"), as the smallest identifiable TJ-like unit that occurs in most, perhaps all strata. We have also determined the specific distributions of TJ proteins in the cell layers of squamous cell metaplasias of the human bronchial tract. Moreover, we show that the occludin-related tetraspanin protein tricellulin-[alpha] connects and seals the membranes of adjacent "three corner" cell structures of the uppermost layer in keratinocytes growing in culture. We hypothesize the possible occurrence of tricellulin-[beta] in more basal cell layers of keratinocyte cultures and the general occurrence of different tricellulin splice forms in stratified epithelia in situ, and discuss the possible functions of TJ proteins in stratified epithelia and tumors derived therefrom.}, author = {Schl\"{u}ter, Holger and Moll, Ingrid and Wolburg, Hartwig and Franke, Werner W. }, booktitle = {Keratinocytes}, citeulike-article-id = {2943643}, doi = {10.1016/j.ejcb.2007.01.001}, journal = {European Journal of Cell Biology}, keywords = {cgn, epithelium, tight-junction}, month = {December}, number = {11-12}, pages = {645--655}, posted-at = {2008-06-30 11:34:03}, priority = {2}, title = {The different structures containing tight junction proteins in epidermal and other stratified epithelial cells, including squamous cell metaplasia}, url = {http://dx.doi.org/10.1016/j.ejcb.2007.01.001}, volume = {86}, year = {2007} }

TY - JOUR ID - citeulike:2943643 L3 - citeulike-article-id:2943643 TI - The different structures containing tight junction proteins in epidermal and other stratified epithelial cells, including squamous cell metaplasia T2 - Keratinocytes JF - European Journal of Cell Biology VL - 86 IS - 11-12 SP - 645 EP - 655 N2 - In stratified squamous epithelia constituent proteins of tight junctions (TJs) are not restricted to the zonula occludens-related structures of the uppermost living cell layer such as the stratum granulosum of the epidermis but TJ membrane proteins such as occludin and certain members of the claudin family as well as TJ plaque proteins, notably cingulin and protein ZO-1, have also been identified by immunofluorescence and immunoelectron microscopy in more basal layers where they form special cell-cell-connecting structures such as the "lamellated" and the "sandwich" junctions. In the present study, we describe another TJ protein-containing structure, the very small puncta occludentia ("stud junctions"), as the smallest identifiable TJ-like unit that occurs in most, perhaps all strata. We have also determined the specific distributions of TJ proteins in the cell layers of squamous cell metaplasias of the human bronchial tract. Moreover, we show that the occludin-related tetraspanin protein tricellulin-[alpha] connects and seals the membranes of adjacent "three corner" cell structures of the uppermost layer in keratinocytes growing in culture. We hypothesize the possible occurrence of tricellulin-[beta] in more basal cell layers of keratinocyte cultures and the general occurrence of different tricellulin splice forms in stratified epithelia in situ, and discuss the possible functions of TJ proteins in stratified epithelia and tumors derived therefrom. KW - cgn KW - epithelium KW - tight-junction AU - Schlüter, Holger AU - Moll, Ingrid AU - Wolburg, Hartwig AU - Franke, Werner PY - 2007/12/03/ UR - http://dx.doi.org/10.1016/j.ejcb.2007.01.001 ER -