Distribution of inositol-1,4,5-trisphosphate receptor isotypes and ryanodine receptor isotypes during maturation of the rat hippocampus

@article{citeulike:2090763, abstract = {Activation of inositol-1,4,5-trisphosphate receptors (InsP3Rs) and ryanodine receptors (RyRs) can lead to the release of Ca2+ from intracellular stores and propagating Ca2+ waves. Previous studies of these proteins in neurons have focused on their distribution in adult tissue, whereas, recent functional studies have examined neural tissue extracted from prenatal and young postnatal animals. In this study we examined the distribution of InsP3R isotypes 1-3 and RyR isotypes 1-3 in rat hippocampus during postnatal maturation, with a focus on InsP3R1 because it is most prominent in the hippocampus. InsP3R1 was observed in pyramidal cells and granule cells, InsP3R2 immunoreactivity was observed in perivascular astrocytes and endothelial cells, and InsP3R3 immunoreactivity was detected in axon terminals located in stratum pyramidale of CA1 and microvessels in stratum radiatum. RyR1 immunolabeling was enriched in CA1, RyR2 was most intense in CA3 and the dentate gyrus, and RyR3 immunolabeling was detected in all subfields of the hippocampus, but was most intense in stratum lacunosum-moleculare. During maturation from 2 to 10 weeks of age there was a shift in InsP3R1 immunoreactivity from a high density in the proximal apical dendrites to a uniform distribution along the dendrites. Independent of age, InsP3R1 immunoreactivity was observed to form clusters within the primary apical dendrite and at dendritic bifurcations of pyramidal neurons. As CA1 pyramidal neurons matured, InsP3R1 was often co-localized with the Ca2+ binding protein calbindin D-28k. In contrast, InsP3R1 immunolabel was never co-localized with calbindin D-28k immunopositive interneurons located outside of stratum pyramidale or with parvalbumin, typically found in hippocampal basket cells, suggesting that InsP3R1s do not play a role in internal Ca2+ release in these interneurons. These findings should help to interpret past functional studies and inform future studies examining the characteristics and consequences of InsP3R-mediated internal Ca2+ release and Ca2+ waves in hippocampal neurons.}, author = {Hertle, D. N. and Yeckel, M. F. }, citeulike-article-id = {2090763}, doi = {10.1016/j.neuroscience.2007.09.058}, journal = {Neuroscience}, keywords = {hippocampus, ip3, ryr}, month = {December}, number = {3}, pages = {625--638}, posted-at = {2007-12-11 17:13:17}, priority = {4}, title = {Distribution of inositol-1,4,5-trisphosphate receptor isotypes and ryanodine receptor isotypes during maturation of the rat hippocampus}, url = {http://dx.doi.org/10.1016/j.neuroscience.2007.09.058}, volume = {150}, year = {2007} }

TY - JOUR ID - citeulike:2090763 L3 - citeulike-article-id:2090763 TI - Distribution of inositol-1,4,5-trisphosphate receptor isotypes and ryanodine receptor isotypes during maturation of the rat hippocampus JF - Neuroscience VL - 150 IS - 3 SP - 625 EP - 638 N2 - Activation of inositol-1,4,5-trisphosphate receptors (InsP3Rs) and ryanodine receptors (RyRs) can lead to the release of Ca2+ from intracellular stores and propagating Ca2+ waves. Previous studies of these proteins in neurons have focused on their distribution in adult tissue, whereas, recent functional studies have examined neural tissue extracted from prenatal and young postnatal animals. In this study we examined the distribution of InsP3R isotypes 1-3 and RyR isotypes 1-3 in rat hippocampus during postnatal maturation, with a focus on InsP3R1 because it is most prominent in the hippocampus. InsP3R1 was observed in pyramidal cells and granule cells, InsP3R2 immunoreactivity was observed in perivascular astrocytes and endothelial cells, and InsP3R3 immunoreactivity was detected in axon terminals located in stratum pyramidale of CA1 and microvessels in stratum radiatum. RyR1 immunolabeling was enriched in CA1, RyR2 was most intense in CA3 and the dentate gyrus, and RyR3 immunolabeling was detected in all subfields of the hippocampus, but was most intense in stratum lacunosum-moleculare. During maturation from 2 to 10 weeks of age there was a shift in InsP3R1 immunoreactivity from a high density in the proximal apical dendrites to a uniform distribution along the dendrites. Independent of age, InsP3R1 immunoreactivity was observed to form clusters within the primary apical dendrite and at dendritic bifurcations of pyramidal neurons. As CA1 pyramidal neurons matured, InsP3R1 was often co-localized with the Ca2+ binding protein calbindin D-28k. In contrast, InsP3R1 immunolabel was never co-localized with calbindin D-28k immunopositive interneurons located outside of stratum pyramidale or with parvalbumin, typically found in hippocampal basket cells, suggesting that InsP3R1s do not play a role in internal Ca2+ release in these interneurons. These findings should help to interpret past functional studies and inform future studies examining the characteristics and consequences of InsP3R-mediated internal Ca2+ release and Ca2+ waves in hippocampal neurons. KW - hippocampus KW - ip3 KW - ryr AU - Hertle, DN AU - Yeckel, MF PY - 2007/12/12/ UR - http://dx.doi.org/10.1016/j.neuroscience.2007.09.058 ER -