Productive infection of human immunodeficiency virus type 1 in dendritic cells requires fusion-mediated viral entry

@article{citeulike:2805568, abstract = {Human immunodeficiency virus type 1 (HIV-1) enters dendritic cells (DCs) through endocytosis and viral receptor-mediated fusion. Although endocytosis-mediated HIV-1 entry can generate productive infection in certain cell types, including human monocyte-derived macrophages, productive HIV-1 infection in DCs appears to be dependent on fusion-mediated viral entry. It remains to be defined whether endocytosed HIV-1 in DCs can initiate productive infection. Using HIV-1 infection and cellular fractionation assays to measure productive viral infection and entry, here we show that HIV-1 enters monocyte-derived DCs predominately through endocytosis; however, endocytosed HIV-1 cannot initiate productive HIV-1 infection in DCs. In contrast, productive HIV-1 infection in DCs requires fusion-mediated viral entry. Together, these results provide functional evidence in understanding HIV-1 cis-infection of DCs, suggesting that different pathways of HIV-1 entry into DCs determine the outcome of viral infection.}, author = {Janas, Alicia M. and Dong, Chunsheng and Wang, Jian-Hua and Wu, Li }, citeulike-article-id = {2805568}, doi = {10.1016/j.virol.2008.01.044}, journal = {Virology}, keywords = {entry, fusion, hiv, macrophage}, month = {June}, number = {2}, pages = {442--451}, posted-at = {2008-05-16 17:52:01}, priority = {2}, title = {Productive infection of human immunodeficiency virus type 1 in dendritic cells requires fusion-mediated viral entry}, url = {http://dx.doi.org/10.1016/j.virol.2008.01.044}, volume = {375}, year = {2008} }

TY - JOUR ID - citeulike:2805568 L3 - citeulike-article-id:2805568 TI - Productive infection of human immunodeficiency virus type 1 in dendritic cells requires fusion-mediated viral entry JF - Virology VL - 375 IS - 2 SP - 442 EP - 451 N2 - Human immunodeficiency virus type 1 (HIV-1) enters dendritic cells (DCs) through endocytosis and viral receptor-mediated fusion. Although endocytosis-mediated HIV-1 entry can generate productive infection in certain cell types, including human monocyte-derived macrophages, productive HIV-1 infection in DCs appears to be dependent on fusion-mediated viral entry. It remains to be defined whether endocytosed HIV-1 in DCs can initiate productive infection. Using HIV-1 infection and cellular fractionation assays to measure productive viral infection and entry, here we show that HIV-1 enters monocyte-derived DCs predominately through endocytosis; however, endocytosed HIV-1 cannot initiate productive HIV-1 infection in DCs. In contrast, productive HIV-1 infection in DCs requires fusion-mediated viral entry. Together, these results provide functional evidence in understanding HIV-1 cis-infection of DCs, suggesting that different pathways of HIV-1 entry into DCs determine the outcome of viral infection. KW - entry KW - fusion KW - hiv KW - macrophage AU - Janas, Alicia AU - Dong, Chunsheng AU - Wang, Jian-Hua AU - Wu, Li PY - 2008/06/05/ UR - http://dx.doi.org/10.1016/j.virol.2008.01.044 ER -