Cannabidiolic-acid synthase, the chemotype-determining enzyme in the fiber-type Cannabis sativa.

by: F Taura, S Sirikantaramas, Y Shoyama, K Yoshikai, Y Shoyama, S Morimoto

FEBS letters, Vol. 581, No. 16. (26 June 2007), pp. 2929-2934.

View FullText article

DOI, Pubmed, Hubmed

Reviews [Write a review of this article]

There are no reviews of this article

Find related articles from these CiteULike users

xico

Find related articles with these CiteULike tags

acid, cannabidiol, cannabidiolic, cannabis, cbd-synthase, cdb, chemotype, fiber, hemp, marijuana, psychoactivity, sativa, synthase, thc

Abstract

Cannabidiolic-acid (CBDA) synthase is the enzyme that catalyzes oxidative cyclization of cannabigerolic-acid into CBDA, the dominant cannabinoid constituent of the fiber-type Cannabis sativa. We cloned a novel cDNA encoding CBDA synthase by reverse transcription and polymerase chain reactions with degenerate and gene-specific primers. Biochemical characterization of the recombinant enzyme demonstrated that CBDA synthase is a covalently flavinylated oxidase. The structural and functional properties of CBDA synthase are quite similar to those of tetrahydrocannabinolic-acid (THCA) synthase, which is responsible for the biosynthesis of THCA, the major cannabinoid in drug-type Cannabis plants.

@article{citeulike:2800291, abstract = {Cannabidiolic-acid (CBDA) synthase is the enzyme that catalyzes oxidative cyclization of cannabigerolic-acid into CBDA, the dominant cannabinoid constituent of the fiber-type Cannabis sativa. We cloned a novel cDNA encoding CBDA synthase by reverse transcription and polymerase chain reactions with degenerate and gene-specific primers. Biochemical characterization of the recombinant enzyme demonstrated that CBDA synthase is a covalently flavinylated oxidase. The structural and functional properties of CBDA synthase are quite similar to those of tetrahydrocannabinolic-acid (THCA) synthase, which is responsible for the biosynthesis of THCA, the major cannabinoid in drug-type Cannabis plants.}, address = {Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan. taura@phar.kyushu-u.ac.jp <taura@phar.kyushu-u.ac.jp>}, author = {Taura, F. and Sirikantaramas, S. and Shoyama, Y. and Yoshikai, K. and Shoyama, Y. and Morimoto, S. }, citeulike-article-id = {2800291}, doi = {10.1016/j.febslet.2007.05.043}, issn = {0014-5793}, journal = {FEBS letters}, keywords = {acid, cannabidiol, cannabidiolic, cannabis, cbd-synthase, cdb, chemotype, fiber, hemp, marijuana, psychoactivity, sativa, synthase, thc}, month = {June}, number = {16}, pages = {2929--2934}, posted-at = {2008-05-15 00:36:16}, priority = {2}, title = {Cannabidiolic-acid synthase, the chemotype-determining enzyme in the fiber-type Cannabis sativa.}, url = {http://dx.doi.org/10.1016/j.febslet.2007.05.043}, volume = {581}, year = {2007} }

RIS record

TY - JOUR ID - citeulike:2800291 L3 - citeulike-article-id:2800291 TI - Cannabidiolic-acid synthase, the chemotype-determining enzyme in the fiber-type Cannabis sativa. JF - FEBS letters VL - 581 IS - 16 SP - 2929 EP - 2934 AD - Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan. taura@phar.kyushu-u.ac.jp <taura@phar.kyushu-u.ac.jp> SN - 0014-5793 N2 - Cannabidiolic-acid (CBDA) synthase is the enzyme that catalyzes oxidative cyclization of cannabigerolic-acid into CBDA, the dominant cannabinoid constituent of the fiber-type Cannabis sativa. We cloned a novel cDNA encoding CBDA synthase by reverse transcription and polymerase chain reactions with degenerate and gene-specific primers. Biochemical characterization of the recombinant enzyme demonstrated that CBDA synthase is a covalently flavinylated oxidase. The structural and functional properties of CBDA synthase are quite similar to those of tetrahydrocannabinolic-acid (THCA) synthase, which is responsible for the biosynthesis of THCA, the major cannabinoid in drug-type Cannabis plants. KW - acid KW - cannabidiol KW - cannabidiolic KW - cannabis KW - cbd-synthase KW - cdb KW - chemotype KW - fiber KW - hemp KW - marijuana KW - psychoactivity KW - sativa KW - synthase KW - thc AU - Taura, F AU - Sirikantaramas, S AU - Shoyama, Y AU - Yoshikai, K AU - Shoyama, Y AU - Morimoto, S PY - 2007/06/26/ UR - http://dx.doi.org/10.1016/j.febslet.2007.05.043 ER -

RIS BibTeX