HIV-1 Proviral DNA Excision Using an Evolved Recombinase

by: Indrani Sarkar, Ilona Hauber, Joachim Hauber, Frank Buchholz

Science, Vol. 316, No. 5833. (29 June 2007), pp. 1912-1915.

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Abstract

HIV-1 integrates into the host chromosome and persists as a provirus flanked by long terminal repeats (LTRs). To date, treatment regimens primarily target the virus enzymes or virus-cell fusion, but not the integrated provirus. We report here the substrate-linked protein evolution of a tailored recombinase that recognizes an asymmetric sequence within an HIV-1 LTR. This evolved recombinase efficiently excised integrated HIV proviral DNA from the genome of infected cells. Although a long way from use in the clinic, we speculate that this type of technology might be adapted in future antiretroviral therapies, among other possible uses. 10.1126/science.1141453

@article{citeulike:1421632, abstract = {HIV-1 integrates into the host chromosome and persists as a provirus flanked by long terminal repeats (LTRs). To date, treatment regimens primarily target the virus enzymes or virus-cell fusion, but not the integrated provirus. We report here the substrate-linked protein evolution of a tailored recombinase that recognizes an asymmetric sequence within an HIV-1 LTR. This evolved recombinase efficiently excised integrated HIV proviral DNA from the genome of infected cells. Although a long way from use in the clinic, we speculate that this type of technology might be adapted in future antiretroviral therapies, among other possible uses. 10.1126/science.1141453}, author = {Sarkar, Indrani and Hauber, Ilona and Hauber, Joachim and Buchholz, Frank }, citeulike-article-id = {1421632}, doi = {http://dx.doi.org/10.1126/science.1141453}, journal = {Science}, keywords = {hiv}, month = {June}, number = {5833}, pages = {1912--1915}, posted-at = {2008-01-22 02:54:48}, priority = {2}, title = {HIV-1 Proviral DNA Excision Using an Evolved Recombinase}, url = {http://dx.doi.org/10.1126/science.1141453}, volume = {316}, year = {2007} }

RIS record

TY - JOUR ID - citeulike:1421632 L3 - citeulike-article-id:1421632 TI - HIV-1 Proviral DNA Excision Using an Evolved Recombinase JF - Science VL - 316 IS - 5833 SP - 1912 EP - 1915 N2 - HIV-1 integrates into the host chromosome and persists as a provirus flanked by long terminal repeats (LTRs). To date, treatment regimens primarily target the virus enzymes or virus-cell fusion, but not the integrated provirus. We report here the substrate-linked protein evolution of a tailored recombinase that recognizes an asymmetric sequence within an HIV-1 LTR. This evolved recombinase efficiently excised integrated HIV proviral DNA from the genome of infected cells. Although a long way from use in the clinic, we speculate that this type of technology might be adapted in future antiretroviral therapies, among other possible uses. 10.1126/science.1141453 KW - hiv AU - Sarkar, Indrani AU - Hauber, Ilona AU - Hauber, Joachim AU - Buchholz, Frank PY - 2007/06/29/ UR - http://dx.doi.org/10.1126/science.1141453 ER -

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