M. tuberculosis and M. leprae Translocate from the Phagolysosome to the Cytosol in Myeloid Cells

@article{citeulike:1577089, abstract = {Summary M. tuberculosis and M. leprae are considered to be prototypical intracellular pathogens that have evolved strategies to enable growth in the intracellular phagosomes. In contrast, we show that lysosomes rapidly fuse with the virulent M. tuberculosis- and M. leprae-containing phagosomes of human monocyte-derived dendritic cells and macrophages. After 2 days, M. tuberculosis progressively translocates from phagolysosomes into the cytosol in nonapoptotic cells. Cytosolic entry is also observed for M. leprae but not for vaccine strains such as M. bovis BCG or in heat-killed mycobacteria and is dependent upon secretion of the mycobacterial gene products CFP-10 and ESAT-6. The cytosolic bacterial localization and replication are pathogenic features of virulent mycobacteria, causing significant cell death within a week. This may also reveal a mechanism for MHC-based antigen presentation that is lacking in current vaccine strains.}, author = {van der Wel, Nicole and Hava, David and Houben, Diane and Fluitsma, Donna and van Zon, Maaike and Pierson, Jason and Brenner, Michael and Peters, Peter J. }, citeulike-article-id = {1577089}, doi = {10.1016/j.cell.2007.05.059}, journal = {Cell}, keywords = {microbiology, mycobacteria}, month = {June}, number = {7}, pages = {1287--1298}, posted-at = {2007-08-20 17:33:00}, priority = {4}, title = {M. tuberculosis and M. leprae Translocate from the Phagolysosome to the Cytosol in Myeloid Cells}, url = {http://dx.doi.org/10.1016/j.cell.2007.05.059}, volume = {129}, year = {2007} }

TY - JOUR ID - citeulike:1577089 L3 - citeulike-article-id:1577089 TI - M. tuberculosis and M. leprae Translocate from the Phagolysosome to the Cytosol in Myeloid Cells JF - Cell VL - 129 IS - 7 SP - 1287 EP - 1298 N2 - Summary M. tuberculosis and M. leprae are considered to be prototypical intracellular pathogens that have evolved strategies to enable growth in the intracellular phagosomes. In contrast, we show that lysosomes rapidly fuse with the virulent M. tuberculosis- and M. leprae-containing phagosomes of human monocyte-derived dendritic cells and macrophages. After 2 days, M. tuberculosis progressively translocates from phagolysosomes into the cytosol in nonapoptotic cells. Cytosolic entry is also observed for M. leprae but not for vaccine strains such as M. bovis BCG or in heat-killed mycobacteria and is dependent upon secretion of the mycobacterial gene products CFP-10 and ESAT-6. The cytosolic bacterial localization and replication are pathogenic features of virulent mycobacteria, causing significant cell death within a week. This may also reveal a mechanism for MHC-based antigen presentation that is lacking in current vaccine strains. KW - microbiology KW - mycobacteria AU - van der Wel, Nicole AU - Hava, David AU - Houben, Diane AU - Fluitsma, Donna AU - van Zon, Maaike AU - Pierson, Jason AU - Brenner, Michael AU - Peters, Peter PY - 2007/06/29/ UR - http://dx.doi.org/10.1016/j.cell.2007.05.059 ER -