Rapid selection of accessible and cleavable sites in RNA by Escherichia coli RNase P and random external guide sequences

@article{citeulike:2390472, abstract = {A method of inhibiting the expression of particular genes by using external guide sequences (EGSs) has been improved in its rapidity and specificity. Random EGSs that have 14-nt random sequences are used in the selection procedure for an EGS that attacks the mRNA for a gene in a particular location. A mixture of the random EGSs, the particular target RNA, and RNase P is used in the diagnostic procedure, which, after completion, is analyzed in a gel with suitable control lanes. Within a few hours, the procedure is complete. The action of EGSs designed by an older method is compared with EGSs designed by the random EGS method on mRNAs from two bacterial pathogens. 10.1073/pnas.0711977105}, author = {Lundblad, Eirik W. and Xiao, Gaoping and Ko, Jae-Hyeong and Altman, Sidney }, citeulike-article-id = {2390472}, doi = {10.1073/pnas.0711977105}, journal = {Proceedings of the National Academy of Sciences}, keywords = {disease, ecoli, rna, rnasep, selection, sequence}, month = {February}, pages = {0711977105+}, posted-at = {2008-02-17 10:56:28}, priority = {4}, title = {Rapid selection of accessible and cleavable sites in RNA by Escherichia coli RNase P and random external guide sequences}, url = {http://dx.doi.org/10.1073/pnas.0711977105}, year = {2008} }

TY - JOUR ID - citeulike:2390472 L3 - citeulike-article-id:2390472 TI - Rapid selection of accessible and cleavable sites in RNA by Escherichia coli RNase P and random external guide sequences JF - Proceedings of the National Academy of Sciences SP - 0711977105 N2 - A method of inhibiting the expression of particular genes by using external guide sequences (EGSs) has been improved in its rapidity and specificity. Random EGSs that have 14-nt random sequences are used in the selection procedure for an EGS that attacks the mRNA for a gene in a particular location. A mixture of the random EGSs, the particular target RNA, and RNase P is used in the diagnostic procedure, which, after completion, is analyzed in a gel with suitable control lanes. Within a few hours, the procedure is complete. The action of EGSs designed by an older method is compared with EGSs designed by the random EGS method on mRNAs from two bacterial pathogens. 10.1073/pnas.0711977105 KW - disease KW - ecoli KW - rna KW - rnasep KW - selection KW - sequence AU - Lundblad, Eirik AU - Xiao, Gaoping AU - Ko, Jae-Hyeong AU - Altman, Sidney PY - 2008/02/08/ UR - http://dx.doi.org/10.1073/pnas.0711977105 ER -